Techniques in Molecular Biology

Volume 2

  • John M. Walker
  • Wim Gaastra

Table of contents

  1. Front Matter
    Pages i-iv
  2. Elaine L. V. Harris
    Pages 1-31
  3. D. T. Elmore
    Pages 32-54
  4. S. J. Burton
    Pages 55-81
  5. John N. Wood
    Pages 98-110
  6. Wim Gaastra, Hans E. N. Bergmans
    Pages 111-126
  7. Elliot B. Gingold
    Pages 140-158
  8. Jeremy W. Dale
    Pages 159-177
  9. Paul D. van Helden, C. Louise Olliver
    Pages 178-186
  10. Roger F. L. James, Frank G. Grosveld
    Pages 187-202
  11. Robert J. Slater
    Pages 203-227
  12. Bé Wieringa
    Pages 251-273
  13. Stephen A. Boffey
    Pages 288-295
  14. Robert Harr, Petter Gustafsson
    Pages 296-317
  15. Back Matter
    Pages 318-332

About this book


The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro­ duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con­ tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro­ duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method­ ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin­ ology, a glossary of terms has been included.


DNA ELISA biology chemistry development enzymes gene transfer genes molecular biology nucleic acid protein transcription

Editors and affiliations

  • John M. Walker
    • 1
  • Wim Gaastra
    • 2
  1. 1.Division of Biological and Environmental SciencesThe Hatfield PolytechnicHatfield, HertfordshireEngland
  2. 2.Department of Infectious Diseases, Section of BacteriologyUniversity of UtrechtUtrechtThe Netherlands

Bibliographic information