Manual of Antisense Methodology

  • Gunther Hartmann
  • Stefan Endres

Part of the Perspectives in Antisense Science book series (DARE, volume 4)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. The Chemistry of Antisense Oligonucleotides

    1. Front Matter
      Pages 1-1
    2. Yogesh S. Sanghvi, Mark Andrade, Rantjit R. Deshmukh, Lars Holmberg, Anthony N. Scozzari, Douglas L. Cole
      Pages 3-23
    3. Bernhard Schu, Heinrich Brinkmeier
      Pages 25-38
  3. Antisense Application in vitro

    1. Front Matter
      Pages 39-39
    2. Martin Bidlingmaier, Anne Krug, Gunther Hartmann
      Pages 41-56
    3. Wolf-Bernhard Offensperger, Christian Thoma, Darius Moradpour, Fritz von Weizsäcker, Hubert E. Blum, Jerzy Madon
      Pages 57-77
    4. Lester Kobzik, Margaret Taylor
      Pages 107-123
  4. Antisense Application in vivo

    1. Front Matter
      Pages 143-143
    2. Rainer Spanagel, Christoph Probst, Deborah C. Mash, T. Skutella
      Pages 145-165
    3. Murray S. Webb, Sandra K. Klimuk, Sean C. Semple, Michael J. Hope
      Pages 167-190
    4. Finbarr E Cotter, Dean Fennell
      Pages 191-201
  5. Back Matter
    Pages 203-205

About this book


In the past few years, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense- based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary.
The Manual of Antisense Methodology emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections: antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment.
The Manual of Antisense Methodology will be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.


Nucleotide cell culture chemistry delivery drugs immunology pharmacology receptor reproduction synthesis

Editors and affiliations

  • Gunther Hartmann
    • 1
  • Stefan Endres
    • 1
  1. 1.University of MunichGermany

Bibliographic information

  • DOI
  • Copyright Information Kluwer Academic Publishers 1999
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-7304-9
  • Online ISBN 978-1-4615-5067-9
  • Series Print ISSN 1566-0389
  • Buy this book on publisher's site