Transgenic Models in Endocrinology

  • Maria G. Castro

Part of the Endocrine Updates book series (ENDO, volume 13)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Harold Gainer, W. Scott Young III
    Pages 25-46
  3. Allan E. Herbison
    Pages 47-58
  4. Gabe E. Owens, Ruth A. Keri, John H. Nilson
    Pages 59-78
  5. Susan E. Murray, Sarah C. Coste, Iris Lindberg, Mary P. Stenzel-Poore
    Pages 143-173
  6. James L. Smart, Malcolm J. Low
    Pages 175-194
  7. Anne David, Daniel Stone, Rachel L. Cowen, Maria G. Castro, Pedro R. Lowenstein
    Pages 195-232
  8. Maria G. Castro, Judith C. Williams, Tom D. Southgate, Joseph Smith-Arica, Daniel Stone, Andres Hurtado-Lorenzo et al.
    Pages 233-256
  9. Back Matter
    Pages 257-265

About this book

Introduction

The dramatic recent expansion in genomic information has motivated the development of new approaches to characterize gene expression and function. A critical issue for both basic and clinical endocrinologists is the physiological role of genes involved in regulating endocrine functions. Transgenic technologies allow the translation of genotypic information into specific phenotypes by using gene overexpression or loss of specific gene functions. Murine functional genomics is thus of central importance in modem biomedical endocrine research. Although mice are at present, the preferred mammalian species for genetic manipulations because of the availability of pluripotent embryonic. stem cells and inbred strains and the relatively low breeding and maintenance costs, transgenic rats have also been generated and used to study endocrine physiology. The two basic techniques used in the creation of transgenic animal models are integration of foreign DNA into a fertilized oocyte by random chromosomal insertion and homologous recombination in embryonic stem cells that are then introduced into zygotes. Transgenic mice and rats serve as sophisticted tools to probe protein function, as models of human disease, and as hosts for the testing of gene replacement and other therapies. Embryonic stem cell libraries for mouse gene deletion are being developed, which will make it possible to generate knockout mice rapidly and without the need to analyze gene structure, construct targeting vectors, and screen embryonic stem cell clones. A novel approach to transgenesis for the expression of DNA within adult differentiated neuroendocrine cells in vivo is using viral vectors.

Keywords

Oxytocin genes growth growth hormone hormone prolactin receptor reproduction transcription tumor

Editors and affiliations

  • Maria G. Castro
    • 1
  1. 1.The University of ManchesterUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4615-1633-0
  • Copyright Information Kluwer Academic Publishers 2001
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-5651-6
  • Online ISBN 978-1-4615-1633-0
  • Series Print ISSN 1566-0729
  • About this book