Membrane Protein Structure

Experimental Approaches

  • Stephen H. White

Part of the Methods in Physiology Series book series (METHPHYS)

Table of contents

  1. Front Matter
    Pages i-x
  2. The Nature of the Membrane Protein Structure Problem

    1. Front Matter
      Pages 1-1
    2. Jean-Luc Popot, Catherine de Vitry, Ariane Atteia
      Pages 41-96
  3. Biochemical and Molecular Biological Approaches: Protein Topology

  4. Direct Structural Approaches

  5. Model and Physicochemical Approaches

    1. Front Matter
      Pages 281-281
    2. G. Andrew Woolley, B. A. Wallace
      Pages 314-334
    3. J. D. Lear, Z. R. Wasserman, W. F. Degrado
      Pages 335-354
  6. Back Matter
    Pages 381-395

About this book


Studies of receptors, ion channels, and other membrane proteins require a solid understanding of the structural principles of these important biomolecules. Membrane protein structure is, however, a very challenging field. The structures of only three types of transmembrane proteins have been determined to moderate or high resolution during the last two decades, a period during which the amino acid sequences of hundreds, if not thousands, of membrane proteins have been reported. As a result, the creation of structural models to serve as guides for studies of receptors, channels, and other membrane proteins has become crucially important. This book has been assembled in order to share the experiences and findings of expert researchers in protein structure and structure-prediction methods as well as membrane biophysics and lipid physical chemistry, whose work establishes the basis for the development of suitable model structures. The reviews presented here emphasize fundamental ideas and provide an entry to the diverse and complex literature. The four major sections deal with the general nature of the membrane protein structure problem, biochemical and molecular biological approaches to protein topology, direct structural methods, and model and physicochemical approaches. The work will be of interest to physiologists, cellular and molecular biologists, biophysicists, and biochemists working on the function of membrane proteins such as receptors, ion channels, and transporters, as well as senior graduate students and independent investigators.


biomolecules biophysics gene membrane physiology protein structure proteins topology

Editors and affiliations

  • Stephen H. White
    • 1
  1. 1.College of Medicine, Department of Physiology and BiophysicsUniversity of California, IrvineIrvineUSA

Bibliographic information