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Recombinant DNA Research and Viruses

Cloning and Expression of Viral Genes

  • Yechiel Becker
  • Julia Hadar

Part of the Developments in Molecular Virology book series (DMVI, volume 5)

Table of contents

  1. Front Matter
    Pages i-x
  2. Cloning and Transfer of Viral Genes

    1. Front Matter
      Pages 1-1
    2. Lee Ratner, Robert C. Gallo, Flossie Wong-Staal
      Pages 15-35
  3. Genetic Engineering Strategy

    1. Front Matter
      Pages 37-37
    2. Rudolf Eichenlaub
      Pages 39-57
    3. Amos B. Oppenheim, Jamal Mahajna, Shoshy Altuvia, Simi Koby, Dina Teff, Hilla Locker-Giladi et al.
      Pages 73-86
  4. Cloning of DNA Virus Genes

    1. Front Matter
      Pages 87-87
    2. Jose M. Almendral, Antonio Talavera, Eladio Viñuela
      Pages 89-102
    3. Andrew Davison, Frazer Rixon
      Pages 103-124
    4. Walter Doerfler, Reinhold Gahlmann, Silvia Stabel, Renate Deuring, Manfred Schulz, Ursula Lichtenberg et al.
      Pages 125-171
    5. Lutz Gissmann, Elisabeth Schwarz
      Pages 173-197
  5. Cloning of Retrovirus DNA

    1. Front Matter
      Pages 199-199
    2. J. Deschamps, A. Burny, R. Kettmann
      Pages 201-204
    3. Eli Keshet, Ahuva Itin, Galit Rotman
      Pages 205-220
  6. Cloning of the DNA form of an RNA Virus Genome

    1. Front Matter
      Pages 221-221
    2. Marc Girard, Michel Dreano, Helena Kopecka, Daniele Benichou, Sylvie Van Der Werf
      Pages 223-244
  7. Cloning of Plant Viruses and Viroid Genes

    1. Front Matter
      Pages 245-245
    2. L. K. Dixon, T. Hohn
      Pages 247-275
    3. Robert A. Owens, Michael C. Kiefer
      Pages 293-304
  8. Expression of Viral Genes in Bacteria

    1. Front Matter
      Pages 305-305
    2. Amos B. Oppenheim, Nurit Katzir, Simi Koby, Hilla Locker-Giladi
      Pages 307-326
    3. Roger J. Watson, John H. Weis, John S. Salstrom, Lynn W. Enquist
      Pages 327-352
    4. D. J. Rowlands, F. Brown
      Pages 353-366
  9. Back Matter
    Pages 367-373

About this book

Introduction

The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high­ lights of recombinant DNA research in the field of animal and plant viruses.

Keywords

antigen gene gene expression retroviruses vaccine virology virus yeast

Editors and affiliations

  • Yechiel Becker
    • 1
  • Julia Hadar
  1. 1.The Hebrew University of JerusalemIsrael

Bibliographic information