Two-Dimensional Electrophoresis and Immunological Techniques

1. Front Matter

   Pages i-xvi

   PDF

2. Basic Theories and Principles of Electrophoresis

   • Bonnie S. Dunbar

   Pages 1-23

3. Applications and Strategies for Use of Polyacrylamide Electrophoresis

   • Bonnie S. Dunbar

   Pages 25-45

4. Sample Preparation for Electrophoresis

   • Bonnie S. Dunbar

   Pages 47-65

5. Protein Detection in Polyacrylamide Gel Electrophoresis

   • Bonnie S. Dunbar

   Pages 67-76

6. Basic Principles of Posttranslational Modification of Proteins and Their Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

   • Bonnie S. Dunbar

   Pages 77-102

7. Isotopic Labeling of Proteins for Electrophoretic Analysis

   • Bonnie S. Dunbar

   Pages 103-116

8. Use of Autoradiography in Polyacrylamide Gel Electrophoresis

   • Bonnie S. Dunbar

   Pages 117-122

9. Strategies for Use of Polyacrylamide Gel Electrophoresis in the Preparation and Characterization of Antibodies

   • Bonnie S. Dunbar

   Pages 123-159

10. Practical Methods for Laboratory Photography

    • Bonnie S. Dunbar

    Pages 161-172

11. Troubleshooting and Artifacts in Two-Dimensional Polyacrylamide Gel Electrophoresis

    • Bonnie S. Dunbar

    Pages 173-195

12. Advances in Technology of High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

    • Bonnie S. Dunbar

    Pages 197-207

13. Back Matter

    Pages 209-372

    PDF

This text is a summary of basic principles and techniques and is dedicated to all those students who have been told by their mentors, "Go forth and do two-dimensional gels and have the results on my desk tomorrow. " No attempt has been made in this text to provide exhaustive lists of references related to basic principles or techniques or to list every com­ pany or supplier involved in this area of research. Nevertheless, it is hoped that sufficient information is given to help a new investigator or student appreciate the complexities but develop sufficient expertise to carry out these techniques successfully. The discussions are designed to instill in basic science and clinical investigators of all levels of expertise an appreciation of the power of combining a variety of techniques as well as to provide basic insight into the theories, complexities, and problems frequently encountered with electrophoretic and immunochemical meth­ ods. Bonnie S. Dunbar Houston v Acknowledgments I wish to thank my students and staff for their patience and support throughout the preparation of this text. I would like to acknowledge my appreciation for my extensive discussions with Dr. David Sammons (Uni­ versity of Arizona) and to Dr. N. L. and Dr. N. G. Anderson and their colleagues (Argonne National Laboratory) for their invaluable advice and suggestions in this area over the years. I thank my research assistant, Ms.

• Tyrosin
• amino acid
• antibody
• blood
• fractionation
• immunization
• immunoglobulin
• phosphorylation
• preparation
• protein
• protein synthesis
• proteins
• reaction
• synthesis
• translation

• Baylor College of Medicine, Houston, USA

  Bonnie S. Dunbar

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