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Gene Quantification

  • François Ferré

Part of the Advanced Biomedical Technologies book series (ABT)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Key Issues, Challenges, and Future Opportunities in Gene Quantification

  3. Methods/Technology Issues

    1. Front Matter
      Pages 17-17
    2. Gene Quantitation Based on PCR Amplification

      1. A. Hayward-Lester, B. S. Chilton, P. A. Underhill, P. J. Oefner, P. A. Doris
        Pages 45-78
      2. Jill M. Kolesar, John G. Kuhn
        Pages 79-96
      3. Lincoln McBride, Ken Livak, Mike Lucero, Federico Goodsaid, Dane Carlson, Junko Stevens et al.
        Pages 97-110
      4. Jean Peccoud, Christine Jacob
        Pages 111-128
      5. Carl Wittwer, Kirk Ririe, Randy Rasmussen
        Pages 129-144
      6. Olivier Lantz, Elizabeth Bonney, Scott Umlauf, Yassine Taoufik
        Pages 145-165
    3. Gene Quantitation Based on Other Target Amplification Systems

      1. Joseph Romano, Paul van de Wiel, Stuart Geiger
        Pages 169-188
    4. Gene Quantitation Based on Signal Amplification

      1. Mark L. Collins, Peter J. Dailey, Lu-Ping Shen, Mickey S. Urdea, Linda J. Wuestehube, Janice A. Kolberg
        Pages 205-223
      2. Attila T. Lörincz, Mariana G. Meijide, James G. Lazar, Abel De La Rosa
        Pages 225-249
  4. Applications

    1. Front Matter
      Pages 251-251
    2. Vladimir Lazar, Ivan Bièche, Michel Bahuau, Yves Giovangrandi, Dominique Bellet, Michel Vidaud
      Pages 253-264
    3. Maninder K. Sidhu, Mei-June Liao, Abbas Rashidbaigi
      Pages 265-276
    4. P. Mickey Williams, Todd Giles, Ayly Tucker, Jane Winer, Chris Heid
      Pages 313-325
    5. Janice A. Kolberg, Douglas N. Ludtke, Lu-Ping Shen, Will Cao, Darrah O’Conner, Mickey S. Urdea et al.
      Pages 327-342
    6. Marston Manthorpe, Jukka Hartikka, H. Lee Vahlsing, Michael Sawdey
      Pages 343-367
  5. Back Matter
    Pages 369-375

About this book

Introduction

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo­ bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num­ bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Keywords

DNA Expression PCR Pathogen Polymerasekettenreaktion Quantitative PCR RNA Termination biology development gene expression genes hybridization membrane transcription

Editors and affiliations

  • François Ferré
    • 1
  1. 1.The Immune Response CorporationCarlsbadUSA

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