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The Polymerase Chain Reaction

  • Kary B. Mullis
  • François Ferré
  • Richard A. Gibbs

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Methodology

    1. Front Matter
      Pages 1-1
    2. Basic Methodology

      1. Kenshi Hayashi
        Pages 3-13
      2. Michael A. Frohman
        Pages 14-37
      3. Jeffrey S. Chamberlain, Joel R. Chamberlain
        Pages 38-46
      4. Janet Embretson, Katherine Staskus, Ernest Retzel, Ashley T. Haase, Peter Bitterman
        Pages 55-64
    3. Quantitation

      1. F. Ferré, A. Marchese, P. Pezzoli, S. Griffin, E. Buxton, V. Boyer
        Pages 67-88
      2. Jamel Chelly, Axel Kahn
        Pages 97-109
      3. Dominic G. Spinella, James M. Robertson
        Pages 110-120
    4. Nonisotopic Detection

      1. Michael J. Heller
        Pages 134-141
      2. Eugene Tu, Edward Jablonski
        Pages 142-150
      3. Norman C. Nelson, Sherrol H. McDonough
        Pages 151-161
    5. Instrumentation

      1. Christian C. Oste
        Pages 165-173
      2. Carl T. Wittwer, Gudrun B. Reed, Kirk M. Ririe
        Pages 174-181
      3. H. R. Garner
        Pages 182-198
    6. Sequencing

      1. Bjorn Andersson, Richard A. Gibbs
        Pages 201-213
      2. Steve S. Sommer, Erica L. Vielhaber
        Pages 214-221
      3. André Rosenthal, Matthias Platzer, D. Stephen Charnock-Jones
        Pages 222-229
  3. Applications

    1. Front Matter
      Pages 231-231
    2. General Applications

      1. Craig Tuerk, Sheela MacDougal-Waugh, Gerald Z. Hertz, Larry Gold
        Pages 233-243
      2. Bruce Budowle, Antti Sajantila, Manfred N. Hochmeister, Catherine T. Comey
        Pages 244-256
      3. Matthias Höss, Oliva Handt, Svante Pääbo
        Pages 257-264
      4. Gavin Dollinger
        Pages 265-274
    3. Genetic Analysis

      1. Didier Montarras, Christian Pinset, Jamel Chelly, Axel Kahn
        Pages 277-294
      2. Bruno W. S. Sobral, Rhonda J. Honeycutt
        Pages 304-319
    4. Assessment of Therapy Effectiveness

    5. Diagnostics

  4. PCR and the World of Business

    1. Front Matter
      Pages 419-419
    2. Ellen Daniell
      Pages 421-426
  5. Back Matter
    Pages 442-458

About this book

Introduction

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc­ ture we had divined from a minimum of experimental data and on theoretical argu­ ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Keywords

biochemistry molecular biology biology chemistry

Editors and affiliations

  • Kary B. Mullis
    • 1
  • François Ferré
    • 2
  • Richard A. Gibbs
    • 3
  1. 1.La JollaUSA
  2. 2.The Immune Response CorporationCarlsbadUSA
  3. 3.Institute for Molecular GeneticsBaylor College of MedicineHoustonUSA

Bibliographic information