Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

  • Julian E. Beesley

Table of contents

  1. Front Matter
    Pages i-xi
  2. Julian E. Beesley
    Pages 1-5
  3. Gillian Brown, Anthony Wong, Julian E. Beesley
    Pages 30-54
  4. Peter Jackson, David Blythe
    Pages 55-72
  5. Alexander D. Hyatt, Terry G. Wise
    Pages 73-107
  6. Chee Gee See
    Pages 138-155
  7. Alfredo Martínez, Yan-gao Man, Steven J. Zullo, Frank Cuttitta
    Pages 156-174
  8. Michael Hurle
    Pages 200-222
  9. Back Matter
    Pages 255-267

About this book


Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.


Antigen FISH PCR biology cell cell biology chemistry hybridization image analysis molecular biology nucleic acid tissue

Editors and affiliations

  • Julian E. Beesley
    • 1
  1. 1.Cell BiologyGlaxo Wellcome Medicines Research CentreStevenage, HertfordshireUK

Bibliographic information