Biological Electron Microscopy

Theory, Techniques, and Troubleshooting

  • Michael J. Dykstra
  • Laura E. Reuss

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Michael J. Dykstra, Laura E. Reuss
    Pages 1-73
  3. Michael J. Dykstra, Laura E. Reuss
    Pages 74-123
  4. Michael J. Dykstra, Laura E. Reuss
    Pages 125-149
  5. Michael J. Dykstra, Laura E. Reuss
    Pages 150-152
  6. Michael J. Dykstra, Laura E. Reuss
    Pages 153-158
  7. Michael J. Dykstra, Laura E. Reuss
    Pages 159-174
  8. Michael J. Dykstra, Laura E. Reuss
    Pages 175-189
  9. Michael J. Dykstra, Laura E. Reuss
    Pages 190-196
  10. Michael J. Dykstra, Laura E. Reuss
    Pages 197-208
  11. Michael J. Dykstra, Laura E. Reuss
    Pages 209-217
  12. Michael J. Dykstra, Laura E. Reuss
    Pages 219-231
  13. Michael J. Dykstra, Laura E. Reuss
    Pages 232-239
  14. Michael J. Dykstra, Laura E. Reuss
    Pages 241-244
  15. Michael J. Dykstra, Laura E. Reuss
    Pages 245-258
  16. Michael J. Dykstra, Laura E. Reuss
    Pages 259-270
  17. Michael J. Dykstra, Laura E. Reuss
    Pages 271-285
  18. Michael J. Dykstra, Laura E. Reuss
    Pages 287-322
  19. Michael J. Dykstra, Laura E. Reuss
    Pages 323-337
  20. Michael J. Dykstra, Laura E. Reuss
    Pages 339-345

About this book

Introduction

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti­ cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru­ ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Keywords

Fixation electron microscopy microscopy scanning electron microscope transmission electron microscopy

Authors and affiliations

  • Michael J. Dykstra
    • 1
  • Laura E. Reuss
    • 1
  1. 1.North Carolina State UniversityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4419-9244-4
  • Copyright Information Kluwer Academic/Plenum Publishers 2003
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-4856-6
  • Online ISBN 978-1-4419-9244-4
  • About this book