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Shiga Toxin-Producing E. coli

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Stephanie Schüller

    1. University of East Anglia, Norwich Medical School, Norwich, UK

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  2. Martina Bielaszewska

    1. National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2291)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Integrated Approach for the Diagnosis of Shiga Toxin-Producing Escherichia coli Infections in Humans

    • Stefano Morabito, Fabio Minelli, Rosangela Tozzoli
    Pages 1-17

  3. Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment

    • Stefanie A. Barth, Rolf Bauerfeind, Christian Berens, Christian Menge
    Pages 19-86

  4. Identification of Shiga Toxin-Producing Escherichia coli Outbreaks Using Whole Genome Sequencing

    • Stefan Bletz, Alexander Mellmann, Barbara Middendorf-Bauchart
    Pages 87-97

  5. Predicting Host Association for Shiga Toxin-Producing E. coli Serogroups by Machine Learning

    • Nadejda Lupolova, Antonia Chalka, David L. Gally
    Pages 99-117

  6. Isolation and Characterization of Shiga Toxin Bacteriophages

    • Lorena Rodríguez-Rubio, Maite Muniesa
    Pages 119-144

  7. Lambda Red–Mediated Recombination in Shiga Toxin-Producing Escherichia coli

    • Kenneth G. Campellone, Alyssa M. Coulter
    Pages 145-162

  8. Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

    • Nicola J. Holden, Kathryn M. Wright, Jacqueline Marshall, Ashleigh Holmes
    Pages 163-175

  9. Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing Escherichia coli Outer Membrane Vesicles

    • Martina Bielaszewska, Lilo Greune, Andreas Bauwens, Petra Dersch, Alexander Mellmann, Christian Rüter
    Pages 177-205

  10. Isolation and Characterization of Shiga Toxin-Associated Microvesicles

    • Annie Willysson, Anne-lie Ståhl, Diana Karpman
    Pages 207-228

  11. Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Receptors

    • Johanna Detzner, Gottfried Pohlentz, Johannes Müthing
    Pages 229-252

  12. Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing Escherichia coli to Epithelial Cells

    • David Ruano-Gallego, Luis Ángel Fernández
    Pages 253-272

  13. Determining Shiga Toxin-Producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber

    • Conor J. McGrath, Stephanie Schüller
    Pages 273-283

  14. Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli–Host Interactions

    • Karol Dokladny, Julie G. In, James Kaper, Olga Kovbasnjuk
    Pages 285-296

  15. Use of the Dynamic TIM-1 Model for an In-Depth Understanding of the Survival and Virulence Gene Expression of Shiga Toxin-Producing Escherichia coli in the Human Stomach and Small Intestine

    • Ophélie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis, Stéphanie Blanquet-Diot
    Pages 297-315

  16. Measuring Effector-Mediated Modulation of Inflammatory Responses to Infection with Enteropathogenic and Shiga Toxin-Producing E. coli

    • Georgina L. Pollock, Cristina Giogha, Elizabeth L. Hartland
    Pages 317-332

  17. Interaction of Bovine Lymphocytes with Products of Shiga Toxin-Producing Escherichia coli

    • Andrew G. Bease, Robin L. Cassady-Cain, Mark P. Stevens
    Pages 333-352

  18. Infection of Immunocompetent Conventional Mice with Shiga Toxin-Producing E. coli: The DSS + STEC Model

    • Gregory Hall, Shinichiro Kurosawa, D. J. Stearns-Kurosawa
    Pages 353-364

  19. Infant Rabbit Model for Studying Shiga Toxin-Producing Escherichia coli

    • Jennifer M. Ritchie
    Pages 365-379

  20. Citrobacter rodentium Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing E. coli Infection

    • Laurice J. Flowers, Shenglan Hu, Anishma Shrestha, Amanda J. Martinot, John M. Leong, Marcia S. Osburne
    Pages 381-397
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Keywords

About this book

This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E. coli  (STEC) and Shiga toxins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Shiga Toxin-Producing E. coli: Methods and Protocols aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis.

Editors and Affiliations

  • University of East Anglia, Norwich Medical School, Norwich, UK

    Stephanie Schüller

  • National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic

    Martina Bielaszewska

Bibliographic Information

  • Book Title: Shiga Toxin-Producing E. coli

  • Book Subtitle: Methods and Protocols

  • Editors: Stephanie Schüller, Martina Bielaszewska

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1339-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1338-2Published: 12 March 2021

  • Softcover ISBN: 978-1-0716-1341-2Published: 13 March 2022

  • eBook ISBN: 978-1-0716-1339-9Published: 11 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 421

  • Number of Illustrations: 26 b/w illustrations, 52 illustrations in colour

  • Topics: Pharmacology/Toxicology, Pathology

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