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Bifidobacteria

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Douwe van Sinderen

    1. Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Marco Ventura

    1. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2278)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Methods for Isolation and Recovery of Bifidobacteria

    • Abelardo Margolles, Lorena Ruiz
    Pages 1-12

  3. Bifidobacterium Transformation

    • Emily C. Hoedt, Roger S. Bongers, Francesca Bottacini, Jan Knol, John MacSharry, Douwe van Sinderen
    Pages 13-19

  4. Isolation of Chromosomal and Plasmid DNA from Bifidobacteria

    • Maria Esteban-Torres, Lorena Ruiz, Rocio Sanchez-Gallardo, Douwe van Sinderen
    Pages 21-29

  5. Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes

    • Gabriele Andrea Lugli
    Pages 31-44

  6. Site-Directed Mutagenesis of Bifidobacterium Strains

    • Kieran James, Douwe van Sinderen
    Pages 45-60

  7. Protocol to Select Bifidobacteria from Fecal and Environmental Samples

    • Giulia Alessandri, Maria Cristina Ossiprandi, Marco Ventura, Douwe van Sinderen
    Pages 61-70

  8. Phageome Analysis of Bifidobacteria-Rich Samples

    • Brian McDonnell, Eoghan Casey, Christian Milani, Gabriele Andrea Lugli, Alice Viappiani, Jennifer Mahony et al.
    Pages 71-85

  9. Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria

    • Grace Ahern, Douwe van Sinderen, Bo Yang, R. Paul Ross, Catherine Stanton
    Pages 87-100

  10. Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides

    • Patricia Ruas-Madiedo
    Pages 101-115

  11. Determination of Bifidobacterial Carbohydrate Utilization Abilities and Associated Metabolic End Products

    • Ana Solopova, Douwe van Sinderen
    Pages 117-129

  12. Model for Murine Gut Colonization by Bifidobacteria

    • Valerio Rossini, Ken Nally
    Pages 131-139

  13. Identification of Bifidobacteria by the Phosphoketolase Assay

    • Monica Modesto, Alice Checcucci, Paola Mattarelli
    Pages 141-148

  14. Determination of Bile Salt Hydrolase Activity in Bifidobacteria

    • Lorena Ruiz, Borja Sánchez, Abelardo Margolles
    Pages 149-155

  15. A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications

    • Lorena Ruiz, Maria Esteban-Torres, Douwe van Sinderen
    Pages 157-182

  16. Metagenomic Analyses of Bifidobacterial Communities

    • Christian Milani
    Pages 183-193

  17. Resistance of Bifidobacteria Toward Antibiotics

    • Miguel Gueimonde, Silvia Arboleya
    Pages 195-208

  18. In Vitro Assessment of Prebiotic Activity

    • Alberto Amaretti, Stefano Raimondi, Nicola Volpi, Maddalena Rossi
    Pages 209-223

  19. Bifidobacterium Genome Assembly and Methylome Analysis Using Pacbio SMRT Sequencing

    • Francesca Bottacini, Douwe van Sinderen
    Pages 225-232

  20. Back Matter

    Pages 233-239
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Keywords

About this book

This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.


Editors and Affiliations

  • Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

    Douwe van Sinderen

  • Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    Marco Ventura

Bibliographic Information

  • Book Title: Bifidobacteria

  • Book Subtitle: Methods and Protocols

  • Editors: Douwe van Sinderen, Marco Ventura

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1274-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1273-6Published: 03 March 2021

  • Softcover ISBN: 978-1-0716-1276-7Published: 04 March 2022

  • eBook ISBN: 978-1-0716-1274-3Published: 02 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 239

  • Number of Illustrations: 8 b/w illustrations, 18 illustrations in colour

  • Topics: Bacteriology, Gastroenterology

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