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Cell Cycle Checkpoints

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. James J. Manfredi

    1. Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2267)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy

    • Yu-Che Cheng, Sheau-Yann Shieh
    Pages 1-6

  3. Detection of Post-translationally Modified p53 by Western Blotting

    • Anna Estevan Barber, David W. Meek
    Pages 7-18

  4. Global Analyses to Identify Direct Transcriptional Targets of p53

    • Matthew D. Galbraith, Zdenek Andrysik, Kelly D. Sullivan, Joaquín M. Espinosa
    Pages 19-56

  5. Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay

    • Stephanie Biber, Lisa Wiesmüller
    Pages 57-71

  6. Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors

    • Chris Lucchesi, Shakur Mohibi, Xinbin Chen
    Pages 73-79

  7. DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids

    • Gerd A. Müller, Kurt Engeland
    Pages 81-90

  8. A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer

    • Kenneth Wu, Zhen-Qiang Pan
    Pages 91-102

  9. DNA Damage Response in Xenopus laevis Cell-Free Extracts

    • Tomas Aparicio Casado, Jean Gautier
    Pages 103-144

  10. Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation

    • Jongkuen Lee, David Dominguez-Sola
    Pages 145-157

  11. Knockdown of Target Genes by siRNA In Vitro

    • Songhee Back, James J. Manfredi
    Pages 159-163

  12. Assaying Cell Cycle Status Using Flow Cytometry

    • Ramy Rahmé
    Pages 165-179

  13. Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues

    • Lois Resnick-Silverman
    Pages 181-190

  14. Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast

    • Rohana Ramalingam, Matthew J. O’Connell
    Pages 191-205

  15. Calreticulin Exposure in Mitotic Catastrophe

    • Lucillia Bezu, Oliver Kepp, Guido Kroemer
    Pages 207-215

  16. Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy

    • Juliette Humeau, Lucillia Bezu, Oliver Kepp, Laura Senovilla, Peng Liu, Guido Kroemer
    Pages 217-226

  17. Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe

    • José Manuel Bravo-San Pedro, Oliver Kepp, Allan Sauvat, Santiago Rello-Varona, Guido Kroemer, Laura Senovilla
    Pages 227-239

  18. Back Matter

    Pages 241-242
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Keywords

About this book

This volume explores the latest advancements in the field of cell cycle checkpoints and their implications for human diseases. Chapters in this book cover topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescence microscopy; DNA affinity purification; knockdown of target genes by siRNA in vitro; and calreticulin exposure in mitotic catastrophe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Cell Cycle Checkpoints: Methods and Protocols is a valuable resource for researcher interested in learning more about this developing field.


Editors and Affiliations

  • Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, USA

    James J. Manfredi

Bibliographic Information

  • Book Title: Cell Cycle Checkpoints

  • Book Subtitle: Methods and Protocols

  • Editors: James J. Manfredi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1217-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2021

  • Hardcover ISBN: 978-1-0716-1216-3Published: 31 March 2021

  • Softcover ISBN: 978-1-0716-1219-4Published: 31 March 2022

  • eBook ISBN: 978-1-0716-1217-0Published: 30 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 242

  • Number of Illustrations: 10 b/w illustrations, 21 illustrations in colour

  • Topics: Cell Biology, Immunology

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