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Homologous Recombination

Methods and Protocols

  • Andrés Aguilera
  • Aura Carreira
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2153)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Sonia I. Barroso, Andrés Aguilera
    Pages 1-8
  3. Erika Casari, Elisa Gobbini, Michela Clerici, Maria Pia Longhese
    Pages 33-45
  4. Yi Zhou, Tanya T. Paull
    Pages 59-69
  5. Domagoj Vugic, Åsa Ehlén, Aura Carreira
    Pages 115-126
  6. Fabio Vanoli, Rohit Prakash, Travis White, Maria Jasin
    Pages 127-143
  7. Olga M. Mazina, Alexander V. Mazin
    Pages 145-167
  8. Raquel Carreira, F. Javier Aguado, Tomas Lama-Diaz, Miguel G. Blanco
    Pages 169-185
  9. Simon Gemble, Mounira Amor-Guéret
    Pages 187-192
  10. Anastasiya Epshtein, Lorraine S. Symington, Hannah L. Klein
    Pages 193-200
  11. Lydia R. Heasley, Nadia M. V. Sampaio, Juan Lucas Argueso
    Pages 201-219
  12. Miyuki Yamaguchi, James E. Haber
    Pages 221-238
  13. Tomas Strucko, Michael Lisby, Uffe Hasbro Mortensen
    Pages 239-252
  14. Fraulin Joseph, So Jung Lee, Eric Edward Bryant, Rodney Rothstein
    Pages 253-265
  15. Yan Yun, Masaru Ito, Sumit Sandhu, Neil Hunter
    Pages 267-286
  16. Tatiana García-Muse
    Pages 287-293
  17. Beth Osia, Rajula Elango, Juraj Kramara, Steven A. Roberts, Anna Malkova
    Pages 307-328
  18. Emanuela Tumini, Andrés Aguilera
    Pages 383-393
  19. Marie-Noelle Simon, Dmitri Churikov, Vincent Géli
    Pages 395-402
  20. Coline Arnould, Vincent Rocher, Gaëlle Legube
    Pages 427-438
  21. Román González-Prieto, María J. Cabello-Lobato, Félix Prado
    Pages 447-458
  22. Veronika Altmannova, Lumir Krejci
    Pages 483-502
  23. Dionna Gamble, Yee Fang Hum, Sue Jinks-Robertson
    Pages 503-519
  24. Carl P. Lehmann, Irene Saugar, José Antonio Tercero
    Pages 521-534
  25. Back Matter
    Pages 555-557

About this book

Introduction

This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. 

Keywords

DNA breaks recombination Anaphase Bridges HR repair C. elegans

Editors and affiliations

  • Andrés Aguilera
    • 1
  • Aura Carreira
    • 2
  1. 1.Centro Andaluz de Biologı´a Molecular, y Medicina Regenerativa, CABIMERUniversidad de SevillaSevillaSpain
  2. 2.Institut Curie, PSL Research University, CNRS, UMR3348, F-91405OrsayFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0644-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2021
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0643-8
  • Online ISBN 978-1-0716-0644-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site