© 2020

Animal Influenza Virus

Methods and Protocols

  • Erica Spackman

Part of the Methods in Molecular Biology book series (MIMB, volume 2123)

Table of contents

  1. Front Matter
    Pages i-xii
  2. General

    1. Front Matter
      Pages 1-1
    2. Mary Lea Killian
      Pages 3-10
    3. Erica Spackman, Ioannis Sitaras
      Pages 11-28
    4. Daniel R. Perez, Brittany Seibert, Lucas Ferreri, Chang-Won Lee, Daniela Rajao
      Pages 37-59
  3. Avian Influenza

    1. Front Matter
      Pages 81-81
    2. Rebecca L. Poulson, Justin D. Brown
      Pages 93-112
    3. Erica Spackman, Scott A. Lee
      Pages 123-135
    4. Erica Spackman, Mary Lea Killian
      Pages 177-193
    5. Carol J. Cardona, David A. Halvorson, Jeffrey Hall, Mary J. Pantin-Jackwood, Justin D. Brown
      Pages 195-209

About this book


This third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza, equine influenza, hemagglutination,  genome sequencing, and influenza in other mammals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Animal Influenza Virus: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.


Antigenic Cartography RNA extraction real-time RT-PCR ELISA Microneutralization

Editors and affiliations

  • Erica Spackman
    • 1
  1. 1.Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research ServiceAthensUSA

Bibliographic information

  • Book Title Animal Influenza Virus
  • Book Subtitle Methods and Protocols
  • Editors Erica Spackman
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0345-1
  • Softcover ISBN 978-1-0716-0348-2
  • eBook ISBN 978-1-0716-0346-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 3
  • Number of Pages XII, 454
  • Number of Illustrations 10 b/w illustrations, 46 illustrations in colour
  • Topics Infectious Diseases
  • Buy this book on publisher's site


“The book is targeted to researchers in animal disease, specifically animal influenza viruses. … This book is a useful resource for individuals involved in animal influenza research, specifically those addressing avian, equine, or swine influenza viruses, but has little application for veterinary practitioners and those investigating non-influenza viruses or other disease agents.” (Marcella Ridgway, Doody’s Book Reviews, July 10, 2020)