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Characterization of Plant Viruses

Methods and Protocols

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Book
  • 2.3k Downloads

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 1-5
  3. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 7-21
  4. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 23-27
  5. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 29-31
  6. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 33-38
  7. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 39-43
  8. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 45-56
  9. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 57-60
  10. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 61-64
  11. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 65-67
  12. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 69-75
  13. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 77-82
  14. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 83-87
  15. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 89-93
  16. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 95-98
  17. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 99-103
  18. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 105-109
  19. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 111-115
  20. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 117-123
  21. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 125-131
  22. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 133-168
  23. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 169-171
  24. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 173-183
  25. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 185-190
  26. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 191-198
  27. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 199-205
  28. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 207-213
  29. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 215-221
  30. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 223-237
  31. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 239-283
  32. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 285-290
  33. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 291-297
  34. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 299-302
  35. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 303-321
  36. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 323-345
  37. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 347-356
  38. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 357-367
  39. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 369-376
  40. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 377-381
  41. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 383-387
  42. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 389-395
  43. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 397-420
  44. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 421-428
  45. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 429-438
  46. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 439-447
  47. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 449-463
  48. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 465-477
  49. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 479-489
  50. Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 491-510

About this book

Introduction

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses onin vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Keywords

Plant Virology Virus Characterization Purification Protocols Serological Methods Biotechnological Approaches

Authors and affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0334-5
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0333-8
  • Online ISBN 978-1-0716-0334-5
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site