© 2020

Molecular Toxicology Protocols

  • Phouthone Keohavong
  • Kamaleshwar P. Singh
  • Weimin Gao

Part of the Methods in Molecular Biology book series (MIMB, volume 2102)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Bioinformatics and Biostatistics

    1. Front Matter
      Pages 1-1
    2. Jonathan W. Boyd, Julia A. Penatzer, Nicole Prince, Julie V. Miller, Alice A. Han, Holly N. Currie
      Pages 3-15
    3. Chia-Hsin Liu, Y. Peter Di
      Pages 61-113
  3. Toxicant-Induced Lung Inflammation and Lung Tumors

  4. Analysis of Protein and Gene Expression and Silencing

  5. Gene Promoter Methylation

  6. Detection of Chromosomal and Genome-Wide Damage

    1. Front Matter
      Pages 235-235
    2. Elise Fouquerel, Patricia Opresko
      Pages 237-249

About this book


This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines.


Bioinformatics Next-generation sequencing Genetic toxicology of cancer Toxins and toxicants Gene mutation DNA damage

Editors and affiliations

  • Phouthone Keohavong
    • 1
  • Kamaleshwar P. Singh
    • 2
  • Weimin Gao
    • 3
  1. 1.Department of Environmental and Occupational Health, Graduate School of Public HealthUniversity of PittsburghPittsburghUSA
  2. 2.Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH)Texas Tech UniversityLubbockUSA
  3. 3.Department of Occupational and Environmental Health SciencesSchool of Public Health West Virginia UniversityMorgantownUSA

Bibliographic information

  • Book Title Molecular Toxicology Protocols
  • Editors Phouthone Keohavong
    Kamaleshwar P. Singh
    Weimin Gao
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0222-5
  • Softcover ISBN 978-1-0716-0225-6
  • eBook ISBN 978-1-0716-0223-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 3
  • Number of Pages XIV, 576
  • Number of Illustrations 39 b/w illustrations, 144 illustrations in colour
  • Topics Pharmacology/Toxicology
    Genetics and Genomics
    Human Genetics
  • Buy this book on publisher's site