Molecular Toxicology Protocols

  • Phouthone Keohavong
  • Kamaleshwar P. Singh
  • Weimin Gao

Part of the Methods in Molecular Biology book series (MIMB, volume 2102)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Bioinformatics and Biostatistics

    1. Front Matter
      Pages 1-1
    2. Jonathan W. Boyd, Julia A. Penatzer, Nicole Prince, Julie V. Miller, Alice A. Han, Holly N. Currie
      Pages 3-15
    3. Chia-Hsin Liu, Y. Peter Di
      Pages 61-113
  3. Toxicant-Induced Lung Inflammation and Lung Tumors

  4. Analysis of Protein and Gene Expression and Silencing

  5. Gene Promoter Methylation

  6. Detection of Chromosomal and Genome-Wide Damage

    1. Front Matter
      Pages 235-235
    2. Elise Fouquerel, Patricia Opresko
      Pages 237-249
  7. Analysis of DNA-Adducts and Surrogate Gene Mutations

    1. Front Matter
      Pages 289-289
    2. David H. Phillips, Volker M. Arlt
      Pages 291-302
    3. Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, Robert H. Heflich
      Pages 315-331
    4. Vasily N. Dobrovolsky, Joseph G. Shaddock, Robert H. Heflich
      Pages 333-348
    5. Phouthone Keohavong, Liqiang Xi, Stephen G. Grant
      Pages 349-359
  8. Detection and Characterization of Cancer Gene Mutation

    1. Front Matter
      Pages 371-371
    2. Lifang Zhang, Weimin Gao, Phouthone Keohavong
      Pages 373-394
    3. Meagan B. Myers, Karen L. McKim, Yiying Wang, Malathi Banda, Barbara L. Parsons
      Pages 395-417
  9. Analysis of DNA Damage and Repair Mechanisms

    1. Front Matter
      Pages 439-439
    2. Dawn M. Stults, Michael W. Killen, Paola Marco-Casanova, Andrew J. Pierce
      Pages 441-457
    3. Michael W. Killen, Dawn M. Stults, Paola Marco-Casanova, Andrew J. Pierce
      Pages 459-482
  10. Analysis of Endoplasmic Reticulum Stress and miRNA/RNA Interaction

  11. Back Matter
    Pages 567-576

About this book


This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines.


Bioinformatics Next-generation sequencing Genetic toxicology of cancer Toxins and toxicants Gene mutation DNA damage

Editors and affiliations

  • Phouthone Keohavong
    • 1
  • Kamaleshwar P. Singh
    • 2
  • Weimin Gao
    • 3
  1. 1.Department of Environmental and Occupational Health, Graduate School of Public HealthUniversity of PittsburghPittsburghUSA
  2. 2.Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH)Texas Tech UniversityLubbockUSA
  3. 3.Department of Occupational and Environmental Health SciencesSchool of Public Health West Virginia UniversityMorgantownUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0222-5
  • Online ISBN 978-1-0716-0223-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site