Immobilization of Enzymes and Cells

Methods and Protocols

  • Jose M. Guisan
  • Juan M. Bolivar
  • Fernando López-Gallego
  • Javier Rocha-Martín

Part of the Methods in Molecular Biology book series (MIMB, volume 2100)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Jose M. Guisan, Fernando López-Gallego, Juan M. Bolivar, Javier Rocha-Martín, Gloria Fernandez-Lorente
    Pages 1-26
  3. Jose M. Guisan, Fernando López-Gallego, Juan M. Bolivar, Javier Rocha-Martín, Gloria Fernandez-Lorente
    Pages 83-92
  4. Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martín, Juan M. Bolivar, Cesar Mateo, Jose M. Guisan
    Pages 93-107
  5. Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan et al.
    Pages 109-117
  6. Fernando López-Gallego, Jose M. Guisan, Lorena Betancor
    Pages 119-127
  7. Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego et al.
    Pages 129-141
  8. Lara Trobo-Maseda, Alejandro H. Orrego, María Romero-Fernández, Jose M. Guisan, Javier Rocha-Martín
    Pages 159-174
  9. Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo et al.
    Pages 175-187
  10. Mihaela Badea, Akhtar Hayat, Jean-Louis Marty
    Pages 189-198
  11. José M. Abad, Marcos Pita, Víctor M. Fernández
    Pages 199-209
  12. Daniel Sánchez-deAlcázar, Mantas Liutkus, Aitziber L. Cortajarena
    Pages 211-226
  13. Mikhail V. Zyzin, Pedro Ramos-Cabrer, Susana Carregal-Romero
    Pages 227-241
  14. Donya Valikhani, Juan M. Bolivar, Bernd Nidetzky
    Pages 243-257
  15. Erienne Jackson, Sonali Correa, Lorena Betancor
    Pages 259-270
  16. Victoria Gascón Pérez, Manuel Sánchez-Sánchez
    Pages 271-296
  17. Alejandro H. Orrego, Fernando López-Gallego, Gloria Fernandez-Lorente, Jose M. Guisan, Javier Rocha-Martín
    Pages 297-308
  18. Tanja Consolati, Juan M. Bolivar, Zdenek Petrasek, Jose Berenguer, Aurelio Hidalgo, Jose M. Guisan et al.
    Pages 319-333
  19. Linda Cerofolini, Enrico Ravera, Marco Fragai, Claudio Luchinat
    Pages 363-383
  20. Jorge A. Trelles, Cintia W. Rivero
    Pages 385-394
  21. Albert Espona-Noguera, Jesús Ciriza, Alberto Cañibano-Hernández, Laura Saenz del Burgo, Jose Luis Pedraz
    Pages 395-405
  22. Ignacio de Ory, Gema Cabrera, Martin Ramirez, Ana Blandino
    Pages 407-415
  23. Gorazd Stojkovič, Polona Žnidaršič-Plazl
    Pages 417-426
  24. Alireza Ebrahiminezhad, Seyedeh-Masoumeh Taghizadeh, Younes Ghasemi, Aydin Berenjian
    Pages 427-435
  25. Christian David, Ingeborg Heuschkel, Katja Bühler, Rohan Karande
    Pages 437-452
  26. Nirupama Mallick
    Pages 453-471
  27. Edorta Santos-Vizcaino, Gorka Orive, Jose Luis Pedraz, Rosa Maria Hernandez
    Pages 473-491
  28. Back Matter
    Pages 493-495

About this book


This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme  orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.



Editors and affiliations

  • Jose M. Guisan
    • 1
  • Juan M. Bolivar
    • 2
  • Fernando López-Gallego
    • 3
  • Javier Rocha-Martín
    • 4
  1. 1.CSIC Campus CantoblancoInstituto de CatalisisMadridSpain
  2. 2.Institute of Biotech and Biochemical EngineeringGraz University of TechnologyGrazAustria
  3. 3.CIC biomaGUNEDonostia-San SebastianSpain
  4. 4.Campus UAM-CSICInstitute of CatatalysisMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0214-0
  • Online ISBN 978-1-0716-0215-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site