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MAIT Cells

Methods and Protocols

  • Helen Kaipe
  • Isabelle Magalhaes
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Table of contents

  1. Front Matter
    Pages i-xii
  2. MAIT Cells in Human Tissues and Blood

    1. Front Matter
      Pages 1-1
    2. Isabelle Magalhaes, Martin Solders, Helen Kaipe
      Pages 3-21
    3. Daniela Di Blasi, Alessandro Vacchini, Gennaro De Libero, Lucia Mori
      Pages 23-38
    4. William Rodin, Patrik Sundström, Marianne Quiding Järbrink
      Pages 39-53
    5. Benedikt Strunz, Christine L. Zimmer, Jonna Bister, Martin A. Ivarsson, Niklas K. Björkström
      Pages 71-82
    6. Anna Gibbs, Michal J. Sobkowiak, Johan K. Sandberg, Annelie Tjernlund
      Pages 83-94
  3. Functional Analysis of MAIT Cells

    1. Front Matter
      Pages 95-95
    2. Joachim P. Hagel, Lucy C. Garner, Matthew Bilton, Hema Mehta, Tianqi Leng, Carl-Philipp Hackstein et al.
      Pages 97-124
    3. Mariolina Salio, Vincenzo Cerundolo
      Pages 125-139
    4. Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Timothy S. C. Hinks
      Pages 141-147 Open Access
    5. Wan Rong Sia, Caroline Boulouis, Muhammad Yaaseen Gulam, Andrea Lay Hoon Kwa, Johan K. Sandberg, Edwin Leeansyah
      Pages 149-165
    6. Susanne Jahreis, Sarah Boettcher, Marie von Lilienfeld-Toal
      Pages 167-177
    7. Farhat Parween, Hongwei H. Zhang, Joshua M. Farber
      Pages 179-188
  4. Monitoring and In Vitro Amplification of MAIT Cells

    1. Front Matter
      Pages 189-189
    2. Nicole A. Ladd, Cara A. Froyd, Erin J. Adams
      Pages 191-207
    3. Isabel Bernal, Marco van Ham, Lothar Jänsch
      Pages 209-218
    4. Meghan Cansler, Megan Null, Erin Meermeier, Gwendolyn Swarbrick, David Lewinsohn, Melanie Harriff
      Pages 219-235
  5. MAIT Cells in Murine Models

    1. Front Matter
      Pages 259-259
    2. Timothy S. C. Hinks, Bonnie van Wilgenburg, Huimeng Wang, Liyen Loh, Marios Koutsakos, Katherine Kedzierska et al.
      Pages 261-281 Open Access
    3. Isabelle Nel, Lucie Beaudoin, Agnès Lehuen
      Pages 283-297
    4. Samantha J. Winter, Andreas Krueger
      Pages 299-305
  6. Back Matter
    Pages 307-310

About this book

Introduction

This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.

Chapters 9 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Keywords

microscopy flow cytometry mouse models MAIT heterogeneity MR1 expressing cells

Editors and affiliations

  • Helen Kaipe
    • 1
  • Isabelle Magalhaes
    • 2
  1. 1.Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenClinical Immunology and Transfusion Medicine, Karolinska University HospitalStockholmSweden
  2. 2.Department of Oncology-PathologyKarolinska InstitutetStockholmSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0207-2
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0206-5
  • Online ISBN 978-1-0716-0207-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site