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Animal Cell Biotechnology

Methods and Protocols

  • Ralf Pörtner
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2095)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Cell Line and Media Development

    1. Front Matter
      Pages 1-1
    2. Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, Uwe Jandt
      Pages 3-16
  3. Techniques for Process Development

    1. Front Matter
      Pages 41-41
    2. Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina
      Pages 83-103
    3. Tim Bürgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl et al.
      Pages 105-123
    4. Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, Udo Reichl
      Pages 141-168
    5. Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, Dieter Eibl
      Pages 169-186
  4. Model-Based Techniques for Process Development

    1. Front Matter
      Pages 187-187
    2. Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, Christoph Herwig
      Pages 189-211
    3. Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández Rodríguez, Kim B. Kuchemüller, Johannes Möller et al.
      Pages 213-234
    4. Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller
      Pages 235-249
    5. Tanja Hernández Rodríguez, Björn Frahm
      Pages 251-267
  5. Process Analysis

    1. Front Matter
      Pages 269-269
    2. Olivier Henry, Eric Karengera, Florian Cambay, Gregory De Crescenzo
      Pages 285-293
    3. Mario Lederle, Mircea Tric, Claudio Packi, Tobias Werner, Philipp Wiedemann
      Pages 319-333
    4. Lukas Käßer, Jan Zitzmann, Tanja Grein, Tobias Weidner, Denise Salzig, Peter Czermak
      Pages 335-364
  6. Downstream Techniques

    1. Front Matter
      Pages 365-365
    2. Ricardo J. S. Silva, João P. Mendes, Manuel J. T. Carrondo, Paula M. Marques, Cristina Peixoto
      Pages 367-384
  7. Back Matter
    Pages 397-399

About this book

Introduction

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.


Keywords

Protein Expression MicroReactor System Chip technology process strategies Microbioreactors

Editors and affiliations

  • Ralf Pörtner
    • 1
  1. 1.Institute of Bioprocess and Biosystems EngineeringHamburg University of Technology (TUHH)HamburgGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0191-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0190-7
  • Online ISBN 978-1-0716-0191-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site