Advertisement

Inositol Phosphates

Methods and Protocols

  • Gregory J. Miller
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2091)

Table of contents

  1. Front Matter
    Pages i-x
    PDF
  2. A Short Historical Perspective of Methods in Inositol Phosphate Research
    Stephen B. Shears
    Pages 1-28

  3. Isolation and Measurement

    1. Front Matter
      Pages 29-29
      PDF
    2. Quantitation of Inositol Phosphates by HPLC-ESI-MS
      Quynh H. Duong, Ronald B. Pegg
      Pages 31-37
    3. Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation
      Benjamin L. Turner
      Pages 39-46
    4. Detection of Inositol Phosphates by Split PH Domains
      Reiko Sakaguchi, Shunsuke Tajima, Yasuo Mori, Takashi Morii
      Pages 47-57
    5. Dictyostelium discoideum as a Model to Study Inositol Polyphosphates and Inorganic Polyphosphate
      Yann Desfougères, Adolfo Saiardi
      Pages 59-71

  4. Assays for Function

    1. Front Matter
      Pages 73-73
      PDF
    2. Characterizing Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family
      Lucinda Winward, Rajagopal Sharada Kilari, Stephen T. Safrany
      Pages 75-82
    3. Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells
      Andrew T. Hale, Bradley P. Clarke, John D. York
      Pages 83-92
    4. Back-Pyrophosphorylation Assay to Detect In Vivo InsP7-Dependent Protein Pyrophosphorylation in Mammalian Cells
      Manasa Chanduri, Rashna Bhandari
      Pages 93-105
    5. Analyses of Ligand Binding to IP3 Receptors Using Fluorescence Polarization
      Ana M. Rossi, Colin W. Taylor
      Pages 107-120
    6. Investigating the InsP3 Receptor in Living Cells by Caged InsP3
      Xin Hui, Peter Lipp
      Pages 121-129
    7. Optical Measurement of Synaptic Vesicle Fusion and Its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons
      Sung Hyun Kim
      Pages 131-135
    8. Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate
      Akihiko Tanimura, Satoshi Shuto
      Pages 137-144
    9. Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle–Vesicle Fusion Assay
      Mi Sook Sung, Tae-Young Yoon
      Pages 145-152

  5. Chemical Tools

    1. Front Matter
      Pages 153-153
      PDF
    2. Engineering Protease-Resistant and Highly Active Phytases
      Canfang Niu, Peilong Yang, Bin Yao
      Pages 155-162
    3. Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-Cleaving Tag
      Keehwan Kwon, Marco Biancucci, Patrick J. Woida, Karla J. F. Satchell
      Pages 163-179
  6. Back Matter
    Pages 181-185
    PDF

About this book

Introduction

This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Keywords

Dictyostelium discoidium Enzymatic synthesis affinity probes Cellular delivery complex phosphorylation patterns inositol phosphates

Editors and affiliations

  • Gregory J. Miller
    • 1
  1. 1.Department of ChemistryThe Catholic University of AmericaWashington, DCUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0167-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0166-2
  • Online ISBN 978-1-0716-0167-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site