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Inositol Phosphates

Methods and Protocols

  • Gregory J. Miller
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2091)

Table of contents

  1. Front Matter
    Pages i-x
  2. Isolation and Measurement

    1. Front Matter
      Pages 29-29
    2. Quynh H. Duong, Ronald B. Pegg
      Pages 31-37
    3. Reiko Sakaguchi, Shunsuke Tajima, Yasuo Mori, Takashi Morii
      Pages 47-57
  3. Assays for Function

  4. Chemical Tools

    1. Front Matter
      Pages 153-153
    2. Canfang Niu, Peilong Yang, Bin Yao
      Pages 155-162
    3. Keehwan Kwon, Marco Biancucci, Patrick J. Woida, Karla J. F. Satchell
      Pages 163-179
  5. Back Matter
    Pages 181-185

About this book

Introduction

This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Keywords

Dictyostelium discoidium Enzymatic synthesis affinity probes Cellular delivery complex phosphorylation patterns inositol phosphates

Editors and affiliations

  • Gregory J. Miller
    • 1
  1. 1.Department of ChemistryThe Catholic University of AmericaWashington, DCUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0167-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0166-2
  • Online ISBN 978-1-0716-0167-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site