Targeting Enzymes for Pharmaceutical Development

Methods and Protocols

  • Nikolaos E. Labrou

Part of the Methods in Molecular Biology book series (MIMB, volume 2089)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nikolaos Georgakis, Elisavet Ioannou, Christina Varotsou, Georgios Premetis, Evangelia G. Chronopoulou, Nikolaos E. Labrou
    Pages 41-46
  3. Alanna E. Sorenson, Patrick M. Schaeffer
    Pages 69-85
  4. Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
    Pages 87-118 Open Access
  5. Steven W. Polyak, Rumana Mowla, Henrietta Venter
    Pages 119-130
  6. Jan Schejbal, Roman Řemínek, Zdeněk Glatz
    Pages 147-157
  7. Alanna E. Sorenson, Patrick M. Schaeffer
    Pages 159-166
  8. I. Ayelen Ramallo, Mario O. Salazar, Ricardo L. E. Furlan
    Pages 179-189
  9. Soo-Yeon Kang, Kyeong-Nam Yu, Pranav Joshi, Moo-Yeal Lee
    Pages 191-207
  10. Abraham Ochoa, Frida Trejo, Luis F. Olguín
    Pages 209-233
  11. Evangelia G. Chronopoulou, Christina Varotsou, Nikolaos Georgakis, Georgios Premetis, Elisavet Ioannou, Nikolaos E. Labrou
    Pages 235-243
  12. Baskaran Gunasekaran, Mohd Yunus Shukor
    Pages 245-250
  13. Xiao-Yuan Mao
    Pages 251-256
  14. Piyoosh Sharma, Manish Kumar Tripathi, Sushant Kumar Shrivastava
    Pages 257-286
  15. Rohanah Hussain, Charlotte S. Hughes, Giuliano Siligardi
    Pages C1-C1
  16. Back Matter
    Pages 287-290

About this book


This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols serves as a vital reference for academics and industry professionals working on expanding our understanding of the wide range of important enzyme targets.

The chapter “Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism” is available open access under a Creative Commons Attribution 4.0 International License via


Drug design Drug targets Enzyme inhibitors Biocomputing Enzymatic assays Compound mixtures

Editors and affiliations

  • Nikolaos E. Labrou
    • 1
  1. 1.Department of BiotechnologyAgricultural University of AthensAthensGreece

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0162-4
  • Online ISBN 978-1-0716-0163-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site