About this book
This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols serves as a vital reference for academics and industry professionals working on expanding our understanding of the wide range of important enzyme targets.
The chapter “Enzyme–Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Drug design Drug targets Enzyme inhibitors Biocomputing Enzymatic assays Compound mixtures
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-0716-0163-1
- Copyright Information Springer Science+Business Media, LLC 2020
- Publisher Name Humana, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-0716-0162-4
- Online ISBN 978-1-0716-0163-1
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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