Chimeric Antigen Receptor T Cells

Development and Production

  • Kamilla Swiech
  • Kelen Cristina Ribeiro Malmegrim
  • Virgínia Picanço-Castro

Part of the Methods in Molecular Biology book series (MIMB, volume 2086)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Virgínia Picanço-Castro, Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Dimas Tadeu Covas
    Pages 1-10
  3. Design of Chimeric Antigen Receptors and Vector Production

    1. Front Matter
      Pages 11-11
    2. Nestor F. Leyton-Castro, Marcelo M. Brigido, Andrea Q. Maranhão
      Pages 13-26
    3. Anindya Dasgupta, Stuart Tinch, Kathleen Szczur, Rebecca Ernst, Nathaniel Shryock, Courtney Kaylor et al.
      Pages 27-60
    4. Marlous G. Lana, Bryan E. Strauss
      Pages 61-67
    5. Pablo Diego Moço, Mário Soares de Abreu Neto, Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
      Pages 69-76
  4. CAR-T Cells Generation and Manufacturing

    1. Front Matter
      Pages 85-85
    2. Miliotou N. Androulla, Papadopoulou C. Lefkothea
      Pages 87-117
    3. Leonardo Chicaybam, Luiza Abdo, Martín H. Bonamino
      Pages 131-137
    4. Amanda Mizukami, Kamilla Swiech
      Pages 139-150
  5. CAR-T Cells Characterization and Quality Control

    1. Front Matter
      Pages 179-179
    2. Kenneth Cornetta, Sue Koop, Emily Nance, Kimberley House, Lisa Duffy
      Pages 181-194
    3. Júlia Teixeira Cottas de Azevedo, Amanda Mizukami, Pablo Diego Moço, Kelen Cristina Ribeiro Malmegrim
      Pages 195-201
    4. Robert D. Schwab, Darel Martínez Bedoya, Tiffany R. King, Bruce L. Levine, Avery D. Posey Jr.
      Pages 203-211
    5. Renata Nacasaki Silvestre, Pablo Diego Moço, Virgínia Picanço-Castro
      Pages 213-222
    6. Hugo Calderon, Maksim Mamonkin, Sonia Guedan
      Pages 223-236
    7. Marcelo de Souza Fernandes Pereira, Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
      Pages 237-250
    8. Alba Rodriguez-Garcia, Keisuke Watanabe, Sonia Guedan
      Pages 251-271
  6. Back Matter
    Pages 273-274

About this book


This volume provides comprehensive methods from expert scientists working in the  Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.


Cancer treatment Cytotoxicity Cell manufacturing CAR-T cell CAR-T cell generation

Editors and affiliations

  • Kamilla Swiech
    • 1
  • Kelen Cristina Ribeiro Malmegrim
    • 2
  • Virgínia Picanço-Castro
    • 3
  1. 1.School of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  2. 2.School of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  3. 3.Center for Cell-Based Therapy CTC Regional Blood Center of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0145-7
  • Online ISBN 978-1-0716-0146-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site