Nucleic Acid Detection and Structural Investigations

Methods and Protocols

  • Kira Astakhova
  • Syeda Atia Bukhari

Part of the Methods in Molecular Biology book series (MIMB, volume 2063)

Table of contents

  1. Front Matter
    Pages i-xi
  2. In Vitro Detection

    1. Front Matter
      Pages 1-1
    2. Gabriel Antonio S. Minero, Valentina Cangiano, Jeppe Fock, Francesca Garbarino, Mikkel F. Hansen
      Pages 3-15
    3. Dmitry E. Kireev, Valentina M. Farzan, German A. Shipulin, Vladimir A. Korshun, Timofei S. Zatsepin
      Pages 27-35
    4. D. S. Novopashina, O. A. Semikolenova, A. G. Venyaminova
      Pages 45-56
    5. Sangita Khatri, Elizabeth D. Mellins, Kathryn S. Torok, Syeda Atia Bukhari, Kira Astakhova
      Pages 57-71
    6. Pablo Fagúndez, Gustavo Brañas, Justo Laíz, Juan Pablo Tosar
      Pages 73-83
  3. Nanotechnology and Imaging

    1. Front Matter
      Pages 85-85
    2. Philipp M. G. Löffler, Oliver Ries, Stefan Vogel
      Pages 101-118
    3. M. Leontien van der Bent, Derick G. Wansink, Roland Brock
      Pages 119-138
  4. Biomedical Applications and Big Data

    1. Front Matter
      Pages 155-155
    2. Paula C. G. Onofre-Oliveira, Mariz Vainzof
      Pages 171-180
    3. Nicolas Borisov, Maxim Sorokin, Andrew Garazha, Anton Buzdin
      Pages 189-206
    4. Marianna Zolotovskaia, Maxim Sorokin, Andrew Garazha, Nikolay Borisov, Anton Buzdin
      Pages 207-234
    5. Victor Tkachev, Maxim Sorokin, Andrew Garazha, Nicolas Borisov, Anton Buzdin
      Pages 235-255
    6. Marina Dunaeva, Ger J. M. Pruijn
      Pages 257-268
  5. Back Matter
    Pages 269-270

About this book


This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.


CRISPR-Cas9 STED microscopy RNA nucleotides miRNA in situ hybridization

Editors and affiliations

  • Kira Astakhova
    • 1
  • Syeda Atia Bukhari
    • 2
  1. 1.Department of ChemistryTechnical University of DenmarkKongens LyngbyDenmark
  2. 2.Department of ChemistryTechnical University of DenmarkKongens LyngbyDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0137-2
  • Online ISBN 978-1-0716-0138-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site