Somatic Cell Nuclear Transfer

  • Peter Sutovsky

Part of the Advances in Experimental Medicine and Biology book series (volume 591)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Keith E. Latham, Shaorong Gao, Zhiming Han
    Pages 14-29
  3. Björn Oback, David N. Wells
    Pages 30-57
  4. Kellie L. K. Tamashiro, Randall R. Sakai, Yukiko Yamazaki, Teruhiko Wakayama, Ryuzo Yanagimachi
    Pages 72-83
  5. Jozef Laurincik, Poul Maddox-Hyttel
    Pages 84-92
  6. Josef Fulka Jr., Helena Fulka
    Pages 93-102
  7. Stefan Hiendleder
    Pages 103-116
  8. Christopher Malcuit, Rafael A. Fissore
    Pages 117-131
  9. Back Matter
    Pages 133-135

About this book

Introduction

When I was first approached to edit the present volume, I was somewhat reluc­ tant to undertake this humbling task because most of my own scientific expertise is derived fi'om studies of mammalian fertilization rather than somatic cell nuclear transfer (SCNT). However, in the last ten years, I was very fortunate to train, col­ laborate, and communicate with a number of distinguished "doners," many of whom kindly agreed to contribute to the present volume. This experience made me appre­ ciate that the understanding of cellular and molecular events during and after SCNT is deeply rooted in our knowledge of fertilization of mammalian ovum by the speramatozoon, to which I prefer to refer to as "natural fertilization," not to say that SCNT should be regarded as "unnatural fertilization". Unique cellular events aris­ ing from SCNT have no parallel in natural fertilization and early pre-implantation development. The list includes the requirement of donor cell-nuclear remodeling, disposal of donor cell organelles, the possibility of heteroplasmy (i. e. , presence of mixed mitochondrial genomes) and altered mode of oocyte activation. Thanks to seminal contributions of my distinguished colleagues, the present volume reviews the progress of SCNT technology in major mammalian species including mouse, pig, and cattle, while at the same time, parallels can be drawn and distinctions made between the cellular and molecular basis of nuclear transfer and fertilization.

Keywords

Activation DNA Mammalia Organelle Regulation development phenotype

Editors and affiliations

  • Peter Sutovsky
    • 1
  1. 1.Department of Animal ScienceUniversity of Missouri-ColumbiaColumbiaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-0-387-37754-4
  • Copyright Information Landes Bioscience and Springer Sciencet+Business Media, LLC 2007
  • Publisher Name Springer, New York, NY
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-0-387-37753-7
  • Online ISBN 978-0-387-37754-4
  • Series Print ISSN 0065-2598
  • About this book