Resumen
Rhizoctonia solani Kühn es un hongo fitopatógeno que ataca cultivos de arroz provocando disminución en el rendimiento de sus cosechas. Este trabajo evaluó el comportamiento de un consorcio bacteriano denominado “Lp” como agente biocontrolador del hongo fitopatógeno. Se determinó el efecto antifúngico de Lp frente a R. solani en cultivos duales in vitro; obteniéndose un 62 ± 1% de inhibición de crecimiento del hongo en medio Agar Papa Dextrosa y 58,0 ± 0,5% en agar nutritivo. El efecto antagónico estuvo relacionado a una actividad proteolítica extracelular presentes en sobrenadantes libres de células obtenidos de Lp, lo cual se determinó por zimografía. En pruebas de germinación en condiciones de laboratorio, la aplicación de Lp estimuló el crecimiento de plántulas de arroz. Estudios de campo preliminares indican un incremento de 44% en la producción de grano de arroz en cultivos tratados con Lp. Estos resultados demuestran que este consorcio bacteriano puede ser empleado como agente biocontrolador del hongo fitopatógeno R. solani Kühn.
Abstract
R. solani is a fungal phytopathogen that attacks rice, generating low yields in its crops. This study evaluated the behavior of a bacterial consortium (Lp) as a bio controller agent against the phytopathogen fungus. The antagonist effect, using in vitro Lp/R. solani dual cultures preparated in potato dextrose agar and nutritive broth media, reached inhibitory levels of 62,0 ± 1% and 58,0 ± 0,5% respectively as compared with the growth of the fungus in absence of Lp. Besides, Lp grew in a minimal mineral medium supplemented with R. solani as the sole carbon source. The antagonist effect was related to an extracellular proteolytic activity present in the cell free supernatants obtained from Lp, which was determined by zymography. In germination experiments, previous incubations of rice seeds during 12 h with Lp, increased the size of plantlets and roots compared to the non treated seeds. By Lp treatment of a rice paddy, at Calabozo, Venezuela, an increase of 44% (equivalent 867kg/ha) was obtained respect to a control rice field (without any treatment). The data indicated Lp consortium could be used as a biocontroller agent against R. solani in rice cultivation.
Article PDF
Avoid common mistakes on your manuscript.
Referencias
Abreu, E., Gutiérrez, A., Fontana, H., Cartay, r., Molina, E., Kesteren, A., Guillory, M. (1993). La Agricultura. Componente básico del sistema alimentario Venezolano. Fundación Polar. Caracas-Venezuela. p 500.
Alvarez, L. (1997). Producción de arroz en los llanos occidentales de Venezuela. Edición de la Universidad Ezequiel Zamora. Colección Docencia Universitaria. Barinas-Venezuela. pp 23-26; 170–175.
Amaíz, L. (2003). Identificación y caracterización zimográfica de actividades tipo metaloproteasas extra-celulares de bacterias termófilas y posibles usos en tratamiento de desechos industriales. Trabajo especial de Grado para optar el título de en Química. Facyt-UC. Carabobo (Venezuela). 65 pp.
Barea, J., Pozo, M., Azcón, R., Aguilar, C. (2005). Microbial co-operation in the rhizosphere. J. Exp. Bot. 56(417): 1761–1778.
Brunner, K., Zeilinger, S., Ciliento, R., Woo, S., Lorito, M., Kubi, C., Mach, R. (2005). Improvement of the Fungal Biocontrol Agent to Enhance both Antagonism and Induction of Plant Systemic Disease Resistance. Appl. Environ. Microb. 71(7): 3959–3965.
Dev, N., Dawande, A. (2010) .Biocontrol of soil borne plant pathogen Rhizoctonia solani using Trichoderma spp. and Pseudomonas fluorescens. Asiatic J. Biotechnol. Res. 2010 (01): 39–44.
De Marco J., Félix, C. (2002). Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches broom disease. BMC Biochem. 3: 1–7.
Duffy, B., Keel, C., Défago, G. (2004). Potential Role of Pathogen Signaling in Multitrophyc Plant-Microbe Interactions Envolved in Disease Protection. Appl. Environ. Microb. 70(3): 1836–1842.
Guerrero, Y., Rodríguez, A., Rodríguez, N., Del Valle, M., Lauzardo, A. (2011).Perspectivas del uso de bacterias rizosféricas en el control de Pyricularia grisea (Cooke Sacc.) en el cultivo de arroz (Oryza sativa L.). Rev. Colomb. Biotecnol. 13(1): 16–22.
Kazempour, M. (2004). Biological Control of Rhizoctonia solani, the Casual Agent of Rice Sheath Blight by Antagonistics Bacteria in Greenhouse and Field Conditions. Plant Pathol. J. 3(2): 88–96.
Kloepper, J., Ubana, R., Zehnder, G., Murphy, J., Sikora, E., Fernández, C. (1999). Plant root-bacterial interactions in biological control of soilborne diseases and potencial extensión to systemic and foliar diseases. Plant Aust. Plant Pathol. 28: 21–26.
Krishnan, H., Kang, B., Krishnan, A., Kim, K., Kim, Y. (2007). Rhizobium etli USDA9032 Engineered to Produce a Phenazine Antibiotic Inhibits the Growth Fungal Pathogens but is Impaired in Symbiotic Performance. Appl. Environ. Microb. 73(1): 327–330.
León, M., Yaryura, R., Montecchia, M., Hernández, A., Correa, O., Pacheu, N., Kerber, N., García, A. (2009). Antifungal Activity of Selected Indigenous Pseudomonas and Bacillus from the Soybean Rhizosphere. Int. J. Microb. 2009: 1–9.
Meza, A., Esqueda, M., Gardea, A., Tiznado, M., Calleros, G. (2007). Variabilidad morfológica, patogénica y susceptibilidad a Fungicidas de Rhizoctonia solani aislado de rizósfera de Vitis Vinifera Var. Perlette sedles. Rev. Mex. Mic. 24: 1–7.
Mojica, M., Luna, HA., Sandoval, CF., Pereyra, B., Morales, LH., González, NA., Hernández; CE., Alvarado, OG. (2009). Control biológico de la marchitez del chile (Capsicum annuum L.) por Bacillus thuringiensis. ФYTON. Rev. Int. Bot. Exp. 78:105–110.
Montesinos, E., Bonaterra, A., Badosa, E., Frances, J., Alemany, J., Llorente, I., Moragrega, C. (2002). Plant-microbe interactions and the new biotechnological methods of plant disease control. Int. Microbiol. 5: 169–175.
Nagórska, K., Bikowski, M., Obuchowski, M. (2007). Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochem. Pol. 54(3): 495–508.
Nandakumar, R., Babu, S., Radjacommare, R., Raguchander, T., Samiyappan, R. (2002). Pseudomonas fluorescens mediated antifungal activity against Rhizoctonia solani causing sheath blight in rice. Phytopathol. Mediter. 41: 109–119.
Pal, K., Gardener, B. (2006) .Biological Control of Plant Pathogens. The Plant Health Instruc. pp: 1–25.
Preston, G. (2004).Plant perceptions of plant growth-promoting Pseudomones. The Royal Society. 359: 907–918.
Reinoso, Y., Vaillant, D., Casadesús, V., García e. (2006). Caracterización e Identificación de cepas de los géneros Bacillus, Brevibacillus y Paenibacillus aisladas del suelo con potencialidades para el control biológico de Rhizoctonia solani Kϋhn. Universidad de la Habana. Instituto de investigaciones de Sanidad Vegetal, Cuba. pp 1–6.
Ren, H., Gu, G., Long, J., Yin, Q., Wu, T., Song, T., Zhang, S., Chen, Z., Dong, H. (2006). Combinative effects of a bacterial type-III effector and a biocontrol bacterium on rice growth and disease resistance. J. Biosci. 31(5): 617–627.
Rodríguez, A., Pérez, M., Del Valle, M., Lauzardo, A. (2006).Perspectivas del empleo de rizobacterias como agentes de control biológico en cultivos de importancia económica. Rev. Mex. Fitop. 24 (1): 42–49.
Saad, M. (2006). Destruction of Rhizoctonia solani and Phytophthora capsici Causing Tomato Root-rot by Pseudomonas fluorescences Lytic Enzymes. Res. J. Agricult. Biol. Sci. 2(6): 274–281.
Tarik, M., Sumera, Y., Fauzia, H. (2010). Biological control of potato black scurf by rhizosphere associated bacteria. Braz. J. Microb. 41: 439–451.
Trujillo, I., Díaz, A., Hernández, A., Heydrich, M. (2007). Antagonismo de cepas de Pseudomonas fluorescens y Burkholderia cepacia contra hongos fitopatógenos del arroz y del maíz. Rev. Prot. Veg4. 22(1): 41–46.
Vega, L., Fernández, O. (2001). Avances en el Fomento de Productos Fitosanitarios no-sanitarios. 62: 96–101.
Vespermann, A., Kai, M., Piechulla, B. (2007). Rhizobacterial Volatiles Affect the Growth of Fungi and Arabidopsis thaliana. Appl. Environ. Microb. 73(17): 5639–5641.
Author information
Authors and Affiliations
Corresponding author
Additional information
Open Access:
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Amaíz, L., Vargas, R., Medina, L. et al. Evaluación del efecto antagonista de un consorcio bacteriano sobre Rhizoctonia solani Kühn en cultivos de arroz.. Rev Latinoam Biotecnol Ambient Algal 6, 2 (2015). https://doi.org/10.7603/s40682-015-0002-5
Published:
DOI: https://doi.org/10.7603/s40682-015-0002-5