Resumen
La loosenina es una proteína descrita recientemente que presenta actividad amorfogénica sobre la celulosa. Se aisló a partir del hongo Basidiomiceto Bjerkandera adusta y facilita el desprendimiento de azúcares reductores a partir de fibras celulósicas tratadas previamente con ella y posteriormente expuestas a tratamiento con celulasas. Puede unirse a otros polisacáridos como quitina y xylanos aunque no presenta actividad hidrolítica por sí misma. En un análisis Blast empleando la secuencia de aminoácidos de la loosenina se localizó una secuencia del genoma de Neurospora crassa que mostró 59% de similitud. En el presente trabajo, clonamos, expresamos y caracterizamos parcialmente una posible looenina de N. crassa, debido a que estas proteínas tienen potencial para el pretratamiento de materiales lignocelulósicos.
Abstract
Loosenin is a protein recently described which presents amorphogenic activity on cellulose. It was isolated from the Basidiomycete Bjerkandera adusta and it enhances sugar release from cellulosic fibers treated previously with it and then subjected to cellulase treatment. It can also bind to other polysaccharides like chitin and xylans but presents no hydrolytic activity itself. Blast analysis using the loosenin amino acid sequence retrieved a sequence from the Neurospora crassa genome that showed 59% similarity. In this work, we cloned, expressed and partially characterized a putative loosenin from N. crassa, since these proteins have a potential for pretreatment of lignocellulosic materials.
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Campos-Oliver, A., Quiroz-Castañeda, R., Ortiz-Suri, E. et al. Cloning and expression of a hypothetical Loosenin from Neurospora crassa. Rev Latinoam Biotecnol Ambient Algal 4, 1 (2013). https://doi.org/10.7603/s40682-013-0001-3
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DOI: https://doi.org/10.7603/s40682-013-0001-3