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Laboratory Animal Research

, Volume 34, Issue 4, pp 295–301 | Cite as

Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice

  • Min-Jung Kang
  • Jin-A Choi
  • Joo-Hee Choi
  • Ah-Ra Jang
  • Ji-Yeon Park
  • Jae-Hun Ahn
  • Tae-Sung Lee
  • Dong-Yeon Kim
  • Jong-Hwan ParkEmail author
Open Access
Article

Abstract

Nucleotide-binding domain 1 (Nod1) is a cytosolic receptor that is responsible for the recognition of a bacterial peptidoglycan motif containing meso-diaminophimelic acid. In this study, we sought to identify the role of Nod1 in host defense in vivo against pulmonary infection by multidrug resistant Acinetobacter baumannii. Wildtype (WT) and Nod1-deficient mice were intranasally infected with 3x107 CFU of A. baumannii and sacrificed at 1 and 3 days post-infection (dpi). Bacterial CFUs, cytokines production, histopathology, and mouse β-defensins (mBD) in the lungs of infected mice were evaluated. The production of cytokines in response to A. baumannii was also measured in WT and Nod1-deficient macrophages. The bacterial clearance in the lungs was not affected by Nod1 deficiency. Levels of IL-6, TNF-α, and IL-1β in the lung homogenates were comparable at days 1 and 3 between WT and Nod1-deficient mice, except the TNF-α level at day 3, which was higher in Nod1-deficient mice. There was no significant difference in lung pathology and expression of mBDs (mBD1, 2, 3, and 4) between WT and Nod1-deficient mice infected with A. baumannii. The production of IL-6, TNF-α, and NO by macrophages in response to A baumannii was also comparable in WT and Nod1-deficient mice. Our results indicated that Nod1 does not play an important role in host immune responses against A. baumannii infection.

Keywords

Nucleotide-binding domain 1 (Nod1) peptidoglycan Acinetobacter baumannii 

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Copyright information

© The Author(s) 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://doi.org/creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Min-Jung Kang
    • 1
  • Jin-A Choi
    • 1
  • Joo-Hee Choi
    • 1
  • Ah-Ra Jang
    • 1
  • Ji-Yeon Park
    • 1
  • Jae-Hun Ahn
    • 1
  • Tae-Sung Lee
    • 1
  • Dong-Yeon Kim
    • 1
  • Jong-Hwan Park
    • 1
    Email author
  1. 1.Laboratory Animal Medicine, College of Veterinary MedicineChonnam National UniversityGwangjuKorea

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