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Laboratory Animal Research

, Volume 32, Issue 4, pp 257–266 | Cite as

Establishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation

  • Won-Young Lee
  • Ran Lee
  • Hyuk Song
  • Tai-Young Hur
  • Seunghoon Lee
  • Jiyun Ahn
  • Hyunjhung JhunEmail author
Open Access
Article

Abstract

Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity. The testes and epididymides were collected every week after the induction of artificial cryptorchidism (surgery) for one month. To determine the effect of surgical cryptorchidism, the seminiferous tubule diameter was measured and immunohistochemistry using PGP9.5 and GATA4 antibodies was analyzed. The diameters of the seminiferous tubules of abdominal testes were significantly reduced compared to those of the scrotal testes. Immunohistochemistry results showed that PGP9.5 positive undifferentiated spermatogonia were significantly reduced after surgical cryptorchidism induction, but there were no significant changes in GATA-4 positive sertoli cells. To evaluate the testis function recovery rate, orchiopexy was performed on two dogs after 30 days of bilateral cryptorchidism. In the orchiopexy group, SCP3 positive spermatocytes were detected, and spermatogenesis was recovered 8 weeks after orchiopexy. In this study, we provided optimum experimental conditions and time for surgical preparation of a recipient canine model for SSC transplantation. Additionally, our data will contribute to recipient preparation by using surgically induced cryptorchidism in non-rodent species.

Keywords

Spermatogonial stem cell transplantation cryptorchidism recipient preparation canine orchiopexy 

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Copyright information

© BioMed Central Ltd 2016

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://doi.org/creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Won-Young Lee
    • 1
  • Ran Lee
    • 1
  • Hyuk Song
    • 2
  • Tai-Young Hur
    • 3
  • Seunghoon Lee
    • 3
  • Jiyun Ahn
    • 4
  • Hyunjhung Jhun
    • 5
    Email author
  1. 1.Department of Food Bioscience, RIBHS, College of Biomedical & Health ScienceKonkuk UniversityChungjuKorea
  2. 2.Department of Stem Cell and Regenerative BiologyKonkuk UniversitySeoulKorea
  3. 3.Animal Biotechnology DivisionNational Institute of Animal Science, RDAJeonjuKorea
  4. 4.Metabolism and Nutrition Research GroupKorea Food Research InstituteSeongnamKorea
  5. 5.Research Group of Nutraceuticals for Metabolic SyndromeKorea Food Research InstituteSeongnamKorea

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