Abstract
Exposure to Traffic-Related Air Pollution (TRAP) is a public health concern accountable for several health problems each year in Malaysia. Several studies globally have shown that children in schools near high traffic roads have increased risks for Deoxyribonucleic Acid (DNA) damage due to higher exposure to TRAP. The study aimed to determine the association between TRAP exposure with DNA damage and respiratory symptoms among school children in Selangor state. PM2.5, PM10, Ultrafine Particle (UFP), Carbon Monoxide (CO), Carbon Dioxide (CO2), Nitrogen Dioxide (NO2) and Sulphur Dioxide (SO2) were measured as TRAP. A cross-sectional comparative study was carried out among children in Kajang as exposed group (n=52) and Hulu Langat as comparative group (n=52). A set of standardized and validated questionnaires were used to determine respiratory symptoms and history of exposure. Measurements of indoor and outdoor air pollutants were conducted in schools. Buccal mucosa cells were collected, which then followed by investigation of DNA damage using a comet assay. All pollutants were significantly associated with reported cough and wheezing at p<0.05. Meanwhile, PM2.5, PM10 and UFP were significantly associated with comet tail length at p<0.05. Additionally, comet tail length in the exposed group was significantly higher (35.95±7.93 μm) than those in the comparative group (30.32±8.358 μm), and the difference was significantly different (t=3.450, p=0.001). Children were more likely to have genotoxicity of buccal mucosa if they were exposed to higher levels of ultrafine particles (UFP). This study demonstrated that children in schools near heavy traffic roads have an increased risk for respiratory symptoms and DNA damage due to higher exposure to TRAP. Therefore, this study supports its importance as a risk factor in associations documented between TRAP and respiratory health among children.
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References
Arifuddin, A.A., Jalaludin, J., Hisamuddin, N.H. (2019) Air Pollutants Exposure with Respiratory Symptoms and Lung Function among Primary School Children nearby Heavy Traffic Area in Kajang. Asian Journal of Atmospheric Environment 13(1), 21–29, DOI: 10.5572/ajae.2019.13.1.021.
Carpenter, D.O., Bushkin-Bedient, S. (2013) Exposure to Chemicals and Radiation During Childhood and Risk for Cancer Later in Life. Journal of Adolescent Health 52(5), S21–S29, DOI: 10.1016/J.JADOHEALTH.2013.01.027.
Ceretti, E., Feretti, D., Viola, G.C.V., Zerbini, I., Limina, R.M., Zani, C., Gelatti, U. (2014) DNA Damage in Buccal Mucosa Cells of Pre-School Children Exposed to High Levels of Urban Air Pollutants. PLoS One 9(5), e96524, DOI: 10. 1371/journal.pone.0096524.
Domingues, É.P., Silva, G.G., Oliveira, A.B., Mota, L.M., Santos, V.S.V., de Campos, E.O., Pereira, B.B. (2018) Genotoxic effects following exposure to air pollution in street vendors from a high-traffic urban area. Environmental Monitoring and Assessment 190(4), 215, DOI: 10.1007/s10661-018-6598-2.
Gehring, U., Cyrys, J., Sedlmeir, G., Brunekreef, B., Bellander, T., Fischer, P., Bauer, C.P., Reinhardt, D., Wichmann, H.E., Heinrich, J. (2002) Traffic-related air pollution and respiratory health during the first 2 yrs of life. European Respiratory Journal 19(4), 690–698.
Gyori, B.M., Venkatachalam, G., Thiagarajan, P.S., Hsu, D., Clement, M.-V. (2014) OpenComet: An automated tool for comet assay image analysis. Redox Biology 2, 457–465, DOI: 10.1016/j.redox.2013.12.020.
Huang, H.-B., Lai, C.-H., Chen, G.-W., Lin, Y.-Y., Jaakkola, J.J.K., Liou, S.-H., Wang, S.-L. (2012) Traffic-Related Air Pollution and DNA Damage: A Longitudinal Study in Taiwanese Traffic Conductors. PLoS ONE 7(5), e37412, DOI: 10.1371/journal.pone.0037412.
Kavitha, M., Juliana, J., Abdah, M. (2011) Relationship between Exposure to Particulate Matter and Biomarkers among Bus Drivers in Klang Valley, Malaysia. Health and the Environment Journal 2(2), 1–7. Retrieved from http://www.hej.kk.usm.my/pdf/HEJVol.2No.2/Article01.pdf.
Kim, W.K., Lee, Y.W., Yoon, H.-S. (2014) Associations between outdoor of PM2.5 with cough and wheeze symptoms in asthmatic children in Korea. The Journal of Allergy and Clinical Immunology 133(2), AB98, DOI: 10.1016/j.jaci.2013.12. 364.
Kumaravel, T.S., Jha, A.N. (2006) Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutation Research 605(1–2), 7–16, DOI: 10.1016/j.mrgentox.2006.03.002.
Lodovici, M., Bigagli, E. (2011) Oxidative Stress and Air Pollution Exposure. Journal of Toxicology 2011, 1–9, DOI: 10.11 55/2011/487074.
Madureira, J., Paciência, I., Fernandes, E., de O. (2012) Levels and Indoor-Outdoor Relationships of Size-Specific Particulate Matter in Naturally Ventilated Portuguese Schools. Journal of Toxicology and Environmental Health 75(22–23), 1423–1436, DOI: 10.1080/15287394.2012.721177.
Mohamad Fandi, N.F., Wan Mansor, W.A., Jalaludin, J. (2018) Work exposure to traffic air pollutants (PM10, Benzene, Toluene, and Xylene) and respiratory health implications among urban traffic policemen in Klang Valley, Malaysia. Malaysian Journal of Medicine and Health Sciences 14(SP2), 2636–9346. Retrieved from http://www.medic.upm.edu.my/upload/dokumen/2018120408583509_MJMHS_SP_Nov_2018.pdf.
Muhamad Daud, S.A., Jalaludin, J., Sopian, N.A. (2018) Air pollutants exposure and frequency of micronuclei (MN) among primary school children nearby industrial area. Malaysian Journal of Medicine and Health Sciences 14 (SP2), 2636–9346. Retrieved from http://www.medic.upm.edu.my/upload/dokumen/2018120408575208_MJMHS_SP_Nov_2018.pdf.
Neri, M., Ugolini, D., Bonassi, S., Fucic, A., Holland, N., Knudsen, L.E., Merlo, D.F. (2006) Children’s exposure to environmental pollutants and biomarkers of genetic damage: II. Results of a comprehensive literature search and meta-analysis. Mutation Research 612(1), 14–39, DOI: 10.1016/j.mrrev.2005.04.003.
Nordling, E., Berglind, N., Melén, E., Emenius, G., Hallberg, J., Nyberg, F., Bellander, T. (2008) Traffic-Related Air Pollution and Childhood Respiratory Symptoms, Function and Allergies. Epidemiology 19(3), 401–408, DOI: 10.1097/EDE.0b013e31816a1ce3.
Rawi, N.A.M.N., Jalaludin, J., Chua, P.C. (2015) Indoor air quality and respiratory health among Malay preschool children in Selangor. BioMed Research International 248178, DOI: 10.1155/2015/248178.
Reno, A.L., Brooks, E.G., Ameredes, B.T. (2015) Mechanisms of Heightened Airway Sensitivity and Responses to Inhaled SO2 in Asthmatics. Environmental Health Insights 9(Suppl 1), EHI.S15671, DOI: 10.4137/EHI.S15671.
Sioutas, C., Delfino, R.J., Singh, M. (2005) Exposure assessment for atmospheric ultrafine particles (UFPs) and implications in epidemiologic research. Environ Health Perspect 113(8), 947–955, DOI: 10.1289/ehp.7939.
Tuntawiroon, J., Mahidol, C., Navasumrit, P., Autrup, H., Ruchirawat, M. (2006) Increased health risk in Bangkok children exposed to polycyclic aromatic hydrocarbons from traffic-related sources. Carcinogenesis 28(4), 816–822, DOI: 10. 1093/carcin/bgl175.
Vinzents, P.S., Møller, P., Sørensen, M., Knudsen, L.E., Hertel, O., Jensen, F.P., Loft, S. (2005) Personal exposure to ultrafine particles and oxidative DNA damage. Environmental Health Perspectives 113(11), 1485–1490, DOI: 10.1289/ ehp.7562.
Whyand, T., Hurst, J.R., Beckles, M., Caplin, M.E. (2018) Pollution and respiratory disease: can diet or supplements help? A review. Respiratory Research 19(1), 79, DOI: 10.1186/s12931-018-0785-0.
Yang Razali, N.Y., Latif, M.T., Dominick, D., Mohamad, N., Sulaiman, F.R., Srithawirat, T. (2015) Concentration of particulate matter, CO and CO2 in selected schools in Malaysia. Building and Environment 87, 108–116, DOI: 10.1016/j.buildenv.2015.01.015.
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Ismail, I.N., Jalaludin, J., Bakar, S.A. et al. Association of Traffic-Related Air Pollution (TRAP) with DNA Damage and Respiratory Health Symptoms among Primary School Children in Selangor. Asian J. Atmos. Environ 13, 106–116 (2019). https://doi.org/10.5572/ajae.2019.13.2.106
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DOI: https://doi.org/10.5572/ajae.2019.13.2.106