Abstract
Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich’s ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Evan, G.I. and Vousden, K.H. (2001) Proliferation, cell cycle and apoptosis in cancer. Nature, 411, 342–348.
Kim, S.H. and Choi, K.C. (2013) Anti-cancer effect and underlying mechanism(s) of kaempferol, a phytoestrogen, on the regulation of apoptosis in diverse cancer cell models. Toxicol. Res., 29, 229–234.
Fingrut, O. and Flescher, E. (2002) Plant stress hormones suppress the proliferation and induce apoptosis in human cancer cells. Leukemia, 16, 608–616.
Elmore, S. (2007) Apoptosis: a review of programmed cell death. Toxicol. Pathol., 35, 495–516.
Thornberry, N.A. and Lazebnik, Y. (1998) Caspases: enemies within. Science, 281, 1312–1316.
Dreyer, D.L. and Huey, P.F. (1973) Coumarins of Citrus macroptera. Phytochemistry, 12, 3011–3013.
Abbott, I.A., Leakey, R.R. and Elevitch, C.R. (2006) Traditional Trees of Pacific Islands: Their Culture, Environment, and Use (1st edition), Permanent Agriculture Resources, Holualoa, Hawaii, USA, pp. 283–317.
Paul, S., Islam, M., Tanvir, E.M., Ahmed, R., Das, S., Rumpa, N.E., Hossen, M.S., Parvez, M., Gan, S.H. and Khalil, M.I. (2016) Satkara (Citrus macroptera) fruit protects against acetaminophen-induced hepatorenal toxicity in rats. Evid. Based Complement. Alternat. Med., 2016, 9470954.
Malik, S.K. and Chaudhury, R. (2006) The cryopreservation of embryonic axes of two wild and endangered Citrus species. Plant Genet. Resourc., 4, 204–209.
Rahmatullah, M., Khatun, M.A., Morshed, N., Neogi, P.K., Khan, S.U.A., Hossan, S., Mahal, M.J. and Jahan, R. (2010) A randomized survey of medicinal plants used by folk medicinal healers of Sylhet Division, Bangladesh. Adv. Nat. Appl. Sci., 4, 52–62.
Uddin, N., Hasan, M.R., Hossain, M.M., Hasan, M.M., Roy, A., Islam, T., Hossain, M.M., Faruque, A. and Rana, M.S. (2014) Antioxidant, brine shrimp lethality and antimicrobial activities of methanol and ethyl-acetate extracts of Citrus macroptera Montr. fruit using in vitro assay models. Br. J. Pharm. Res., 4, 1725–1738.
Paul, S., Hossen, M.S., Tanvir, E.M., Islam, M.A., Afroz, R., Ahmmed, I., Saha, M., Gan, S.H. and Khalil, M.I. (2015) Antioxidant properties of Citrus macroptera fruit and its in vivo effects on the liver, kidney and pancreas in wistar rats. Int. J. Pharmacol., 11, 899–909.
Sala, A., Recio, M.D.C., Giner, R.M., Máñez, S., Tournier, H., Schinella, G. and Ríos, J.L. (2002) Anti-inflammatory and antioxidant properties of Helichrysum italicum. J. Pharm. Pharmacol., 54, 365–371.
Hyun, J.H., Kang, J.I., Kim, S.C., Kim, E., Kang, J.H., Kwon, J.M., Park, D.B., Lee, Y.J., Yoo, E.S. and Kang, H.K. (2008) The effects of Crinum asiaticum on the apoptosis induction and the reversal of multidrug resistance in HL-60/MX2. Toxicol. Res., 24, 29–36.
Correia, M.T. and Coelho, L.C. (1995) Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu bean). Appl. Biochem. Biotechnol., 55, 261–273.
Al-Mamun, M.A., Husna, J., Khatun, M., Hasan, R., Kamruzzaman, M., Hoque, K.M., Reza, M.A. and Ferdousi, Z. (2016) Assessment of antioxidant, anticancer and antimicrobial activity of two vegetable species of Amaranthus in Bangladesh. BMC Complement. Altern. Med., 16, 157.
Alam, A.K., Hossain, A.S., Khan, M.A., Kabir, S.R., Reza, M.A., Rahman, M.M., Islam, M.S., Rahman, M.A.A., Rashid, M. and Sadik, M.G. (2016) The antioxidative fraction of white mulberry induces apoptosis through regulation of p53 and NFκB in EAC cells. PLoS ONE, 11, e0167536.
Sa, D.J., Lee, E.J. and Yoo, B.S. (2009) Apoptosis induction by menadione in human promyelocytic leukemia HL-60 cells. Toxicol. Res., 25, 113–118.
Lam, S.K. and Ng, T.B. (2011) Lectins: production and prac tical applications. Appl. Microbiol. Biotechnol., 89, 45–55.
Zhang, Z.T., Peng, H., Li, C.Y., Liu, J.J., Zhou, T.T., Yan, Y.F., Li, Y. and Bao, J.K. (2010) Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis via a caspase-dependent pathway as compared to Ophiopogon japonicus lectin. Phytomedicine, 18, 25–31.
De Mejía, E.G. and Prisecaru, V.I. (2005) Lectins as bioactive plant proteins: a potential in cancer treatment. Crit. Rev. Food Sci. Nutr., 45, 425–445.
Kabir, S.R., Nabi, M.M., Haque, A., Zaman, R.U., Mahmud, Z.H. and Reza, M.A. (2013) Pea lectin inhibits growth of Ehrlich ascites carcinoma cells by inducing apoptosis and G2/M cell cycle arrest in vivo in mice. Phytomedicine, 20, 1288–1296.
Islam, S., Nasrin, S., Khan, M.A., Hossain, A.S., Islam, F., Khandokhar, P., Mollah, M.N., Rashid, M., Sadik, G., Rahman, M.A. and Alam, A.H. (2013) Evaluation of antioxidant and anticancer properties of the seed extracts of Syzygium fruticosum Roxb. growing in Rajshahi, Bangladesh. BMC Complement. Altern. Med., 13, 142.
Pai, K.S.R., Srilatha, P., Suryakant, K., Setty, M.M., Nayak, P.G., Rao, C.M. and Baliga, M.S. (2012) Anticancer activity of Berberis aristata in Ehrlich ascites carcinoma-bearing mice: a preliminary study. Pharm. Biol., 50, 270–277.
Favaloro, B., Allocati, N., Graziano, V., Di Ilio, C. and De Laurenzi, V. (2012) Role of apoptosis in disease. Aging (Albany NY), 4, 330–349.
Author information
Authors and Affiliations
Corresponding author
Additional information
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rights and permissions
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Hasan, M.M., Islam, M.S., Hoque, K.F. et al. Effect of Citrus macroptera Fruit Pulp Juice on Alteration of Caspase Pathway Rendering Anti-Proliferative Activity against Ehrlich’s Ascites Carcinoma in Mice. Toxicol Res. 35, 271–277 (2019). https://doi.org/10.5487/TR.2019.35.3.271
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.5487/TR.2019.35.3.271