Skip to main content
Download PDF

Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts

Toxicological Research volume 33, pages 125–134 (2017)Cite this article

Abstract

The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.

References

  1. Tzaphlidou, M. (2004) The role of collagen and elastin in aged skin: an image processing approach. Micron, 35, 173–177.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  2. Song, K.C., Chang, T.S., Lee, H.J., Kim, J.H., Park, J.H. and Hwang, G.S. (2012) Processed Panax ginseng, sun ginseng increases type I collagen by regulating MMP-1 and TIMP-1 expression in human dermal fibroblasts. J. Ginseng Res., 36, 61–67.

    PubMed  PubMed Central  Article  Google Scholar 

  3. Varani, J., Dame, M.K., Rittie, L., Fligiel, S.E., Kang, S., Fisher, G.J. and Voorhees, J.J. (2006) Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and detective mechanical stimulation. Am. J. Pathol., 168, 1861–1868.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  4. Honda, A., Abe, R., Makino, T., Norisugi, O., Fujita, Y., Watanabe, H., Nishihira, J., Iwakura, Y., Yamagishi, S.I., Shimizu, H. and Shimizu, T. (2008) Interleukin-1β and macrophage migration inhibitory factor (MIF) in dermal fibroblasts mediate UVA-induced matrix metalloproteinase-1expression. J. Dermatol. Sci., 49, 63–72.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  5. Brew, K., Deendayal, D. and Nagase, H. (2000) Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim. Biophys. Acta, 1477, 267–283.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  6. Dasu, M.R., Barrow, R.E., Spies, M. and Herndon, D.N. (2003) Matrix metalloproteinase expression in cytokine stimulated human dermal fibroblasts. Burns, 29, 527–531.

    PubMed  Article  PubMed Central  Google Scholar 

  7. Tokumaru, Y., Fujii, M., Otani, Y., Kameyama, K., Imanishi, Y., Igarashi, N. and Kanzaki, J. (2000) Activation of matrix metalloproteinase-2 in head and neck squamous cell carcinoma: studies of clinical samples and in vitro cell lines co-cultured with fibroblasts. Cancer Lett., 150, 15–21.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  8. Godeau, G. and Hornebeck, W. (1988) Morphometric analysis of the degradation of human skin elastic fibres by human leukocyte elastase (EC 3-4-21-37) and human skin fibroblast elastase (EC 3-4-24). Pathol. Biol., 36, 1133–1138.

    CAS  Google Scholar 

  9. Kim, M.S., Lee, S.R., Rho, H.S., Kim, D.H., Chang, I.S. and Chung, J.H. (2005) The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo. Clin. Chim. Acta, 362, 161–169.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  10. Ha, B.G., Park, M.A. Lee, C.M. and Kim, Y.C. (2015) Antiox-idant activity and anti-wrinkle effects of Aceriphyllum rossii leaf ethanol extract. Toxicol. Res., 31, 363–369.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  11. Kim, S.E., Lee, C.M. and Kim, Y.C. (2016) Anti-wrinkle efficacy of Oenothera laciniata methanol extract in human dermal fibroblasts. J. Invest. Cosmotol., 12, 197–203.

    Article  Google Scholar 

  12. Sheng, Y.X., Li, L., Wang, Q., Guo, H.Z. and Guo, D.A. (2005) Simultaneous determination of gallic acid, albiflorin, paeoniflorin, ferulic acid and benzoic acid in Si-Wu decoction by high-performance liquid chromatography DAD method. J. Pharm. Biomed. Anal., 37, 805–810.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  13. Wen, K.C., Huang, C.Y. and Lu, F.L. (1993) Determination of baicalin and puerarin in traditional Chinese medicinal preparations by high-performance liquid chromatography. J. Chromatogr. A, 631, 241–250.

    CAS  Article  Google Scholar 

  14. Namba, T. (1993) Angelicae Radix, Hoikusha, Osaka, pp. 58–61.

    Google Scholar 

  15. Kim, A.R., Lee, J.J. and Lee, M.Y. (2009) Antioxidative effect of Angelica acutiloba Kitagawa ethanol extract. J. Life Sci., 19, 117–122.

    Article  Google Scholar 

  16. Folin, O. and Denis, W. (1912) On phosphotungstic-phospho-molybdic compounds as color reagents. J. Biol. Chem., 12, 239–243.

    CAS  Google Scholar 

  17. Davis, R., Massey, R.C. and McWeeny, D.J. (1980) The catalysis of the N-nitrosation of secondary amines by nitrosophenols. Food Chem., 6, 115–122.

    Article  Google Scholar 

  18. Blois, M.S. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200.

    CAS  Article  Google Scholar 

  19. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26, 1231–1237.

    CAS  Article  Google Scholar 

  20. Marklund, S. and Marklund, G. (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 469–474.

    CAS  Article  Google Scholar 

  21. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265–275.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Joo, S.S., Park, D., Shin, S., Jeon, J.H., Kim, T.K., Choi, Y.J., Lee, S.H., Kim, J.S., Park, S.K., Hwang, B.Y., Lee, D.I. and Kim, Y.B. (2010) Anti-allergic effects and mechanisms of action of the ethanolic extract of Angelica gigas in dinitrofluo-robenzene-induced inflammation models. Environ. Toxicol. Pharmacol., 30, 127–133.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  23. Wang, N.L., Kiyohara, H., Sakurai, M.H. and Yamada, H. (1999) Antigenic epitope for polyclonal antibody against a complement activating pectin from the roots of Angelica acutiloba Kitagawa. Carbohydr. Polym., 39, 257–264.

    CAS  Article  Google Scholar 

  24. Bickers, D.R. and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol., 126, 2565–2575.

    CAS  Article  Google Scholar 

  25. Scharffetter-Kochanek, K., Brenneisen, P., Wenk, J., Herrmann, G., Ma, W., Kuhr, L., Meewes, C. and Wlaschek, M. (2000) Photoaging of the skin from phenotype to mechanisms. Exp. Gerontol., 35, 307–316.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  26. Miao, Y.P., Wen, R., Aoshima, H. and Zhou, P.G. (2004) Synthesis and antioxidative activity of 2-substituted phenyl-5-(3'-indolyl)-oxazole derivatives. Yao Xue Xue Bao, 39, 37–40.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Park, S.J., Yoon, J.H., Kim, Y.E., Yoon, Y.B. and Kim, J.D. (2011) In vitro antioxidant activity of the aqueous of Angelicae gigas Nakai leaves. Korean J. Food Preserv., 18, 817–823.

    Article  Google Scholar 

  28. Mimura, Y., Ihn, H., Jinnin, M., Asano, Y., Yamane, K. and Tamaki, K. (2006) Epidermal growth factor affects the synthesis and degradation of type I collagen in cultured human dermal fibroblasts. Matrix Biol., 25, 202–212.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  29. Pasquali-Ronchetti, I. and Baccarani-Contri, M. (1997) Elastic fiber during development and aging. Microsc. Res. Tech., 38, 428–435.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  30. Bruckner-Tuderman, L., Schnyder, U.W., Winterhalter, K.H. and Bruckner, P. (1987) Tissue form of type VII collagen from human skin and dermal fibroblasts in culture. Eur. J. Biochem., 165, 607–611.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  31. Di Lullo, G.A., Sweeney, S.M., Körkkö, J., Ala-Kokko, L. and San Antonio, J.D. (2002) Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J. Biol. Chem., 277, 4223–4231.

    Article  CAS  Google Scholar 

  32. Talwar, H.S., Griffiths, C.E., Fisher, G.J., Hamilton, T.A. and Voorhees, J.J. (1995) Reduced type I and type III procollagens in photodamaged adult human skin. J. Invest. Dermatol., 105, 285–290.

    CAS  Article  Google Scholar 

  33. Gordon, M.K. and Hahn, R.A. (2010) Collagens. Cell Tissue Res., 339, 247–257.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  34. Lee, J.H., Lee, S.N., Kim, M.G., Kim, M.H., Kim, H.J., Jo, H.J. and Leem, K.H. (2011) Effects of Angelica gigantis Radix extracts on the collagenase activity and procollagen synthesis in HS68 human fibroblasts and tyrosinase activity. Korea J. Herbol., 26, 29–33.

    Google Scholar 

  35. Borkakoti, N. (1998) Matrix metalloproteases: variations on a theme. Prog. Biophys. Mol. Biol., 70, 73–94.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  36. Herman, M.P., Sukhova, G.K., Kisiel, W., Foster, D., Kehry, M.R., Libby, P. and Schönbeck, U. (2001) Tissue factor pathway inhibitor-2 is a novel inhibitor of matrix metalloprotein-ases with implications for atherosclerosis. J. Clin. Invest., 107, 1117–1126.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  37. Sternlicht, M.D. and Werb, Z. (2001) How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Biol., 17, 463–516.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  38. Hwang, B.M., Noh, E.M., Kim, J.S., Kim, J.M., Hwang, J.K., Kim, H.K., Kang, J.S., Kim, D.S., Chae, H.J., You, Y.O., Kwon, K.B. and Lee, Y.R. (2013) Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB. Int. J. Mol. Med., 31, 477–483.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  39. Kähäri, V.M. and Saarialho-Kere, U. (1997) Matrix metallo-proteinases in skin. Exp. Dermatol., 6, 199–213.

    PubMed  Article  PubMed Central  Google Scholar 

  40. Aimes, R.T. and Quigley, J.P. (1995) Matrix metalloprotein-ase-2 is an interstitial collagenase. Inhibitor-free enzyme catalyzes the cleavage of collagen fibrils and soluble native type I collagen generating the specific 3/4- and 1/4-length fragments. J. Biol. Chem., 270, 5872–5876.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  41. You, M.A., Song, Y.K., Jang, H., Kim, D.M. and Byun, S.Y. (2011) Profiling of skin anti-aging related proteins in human dermal fibroblasts by decursin in Angelica gigas Nakai. Korean J. Chem. Eng., 28, 880–885.

    Article  CAS  Google Scholar 

  42. Hwang, B.M., Noh, E.M., Kim, J.S., Kim, J.M., Hwang, J.K., Kim, H.K., Kang, J.S., Kim, D.S., Chae, H.J., You, Y.O., Kwon, K.B. and Lee, Y.R. (2013) Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB. Int. J. Mol. Med., 31, 477–483.

    CAS  PubMed  Article  PubMed Central  Google Scholar 

Download references

Author information

Affiliations

  1. In Vitro Evaluation Team, Ellead Sin & Bio Research, Osong, Korea

    Min Ah Park

  2. Department of Health & Beauty Science, Gyeongbuk Provincial College, Yecheon, Korea

    Mi Ja Sim

  3. Department of Public Health, Keimyung University, 1095 Dalgubeol-daero, Daegu, 42601, Korea

    Young Chul Kim

Authors
  1. Min Ah Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mi Ja Sim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Young Chul Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Young Chul Kim.

Rights and permissions

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://doi.org/creativecommons.org/licenses/by/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Park, M.A., Sim, M.J. & Kim, Y.C. Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts. Toxicol Res. 33, 125–134 (2017). https://doi.org/10.5487/TR.2017.33.2.125

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.5487/TR.2017.33.2.125

Key words

  • Angelica acutiloba root
  • Antioxidant activity
  • Anti-skin wrinkle
  • Collagen
  • Human dermal fibroblasts