The present study investigated the anti-inflammatory effects of dichloromethane extract of Auricularia auricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide (LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration > 10 μg/m/ (p < 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reduced the expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW 264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethane extract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. The inhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion, the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auricularia auricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.
Abrham, G., Dovrat, S., Bessler, H., Grossman, S., Nir, U. and Bergman, M. (2010). Inhibition of inflammatory cytokine secretion by plant-derived compounds inuviscolide and tomen-tosin: The role of NF-κB and STAT1. The Open Pharmacology Journal, 4, 36–44.
Albina, J.L. and Reichner, J.S. (1998). Role of nitric oxide mediation of macrophage cytotoxicity and apoptosis. Cancer Metastasis Rev., 17,39-53.
Bonizzi, G. and Karin, M. (2004). The two NF-jB activation pathways and their role in innate and adaptive immunity. Trends Immunol, 25, 280–288.
Boucher, J.L., Moali, C. and Tenu, J.R (1999). Nitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization. Cell Mol Life Sci., 55, 1015–1028.
Chowdhury, M.A., Abdellatif, K.R.A., Don, Y., Das, D., Suresh, M.R. and Knaus, E.E. (2009). Synthesis of celecoxib analogues possessing aN-difluoromethyl-l,2-dihydropyrid-2-one s-lipoxyge-nase pharmacophore: Biological evaluation as dual inhibitors of cyclooxygenases and 5-Lipoxygenase with anti-inflammatory activity. J. Med. Chem., 52, 1525–1529.
Duffield, J.S. (2003). The inflammatory macrophage: a story of Jekyll and Hyde. Clin Sci., 104, 27–38.
Francia, C., Rapior, S., Courtecuisse, R. and Siroux, Y. (1999). Current research findings on the effects of selected mushrooms on cardiovascular diseases. International Journal of Medicinal Mushrooms, 1, 169–172.
Hyun, E., Bolla, M., Steinhoff, M., John, L.W., del Soldato, P. and Vergnolle, N. (2004). Anti-inflammatory effects of nitric oxide-releasing hydrocortisone NCX 1022, in a murine model of contact dermatitis. British Journal of Pharmacology, 143, 618–625.
Ikekawa, T, Uehara, N., Maeda, Y., Nakanishi, M. and Fukuoka, F. (1969). Antitumor activity of aqueous extracts of edible mushrooms. Cancer Research, 29, 734–735.
Kiemer, A.K., Muller, C. and Vollmar, A.M. (2002). Inhibition of LPS-induced nitric oxide and TNF-alpha production by alpha-lipoic acid in rat Kupffer cells and in RAW 264.7 murine macrophages. Immunol. Cell. Biol, 80, 550–557.
Krakauer, T. (2004). Molecular therapeutic targets in inflammation, cyclooxygenase and NF-KB. Curr. Drug. Targets., 3, 317–324.
Misaki, A., Kakuta, M., Sasaki, T., Tanaka, M. and Miyaji, H. (1981). Studies on interrelation of structure and antitumor effects of polysaccharides: antitumor action of periodate-modi-fied, branched (1→3)-p-D-glucan of Auricularia auricula-judae, and other polysaccharides containing (1→3)-glycosidic linkages. Carbohydrate Research, 92, 115–129.
Murakami, A., Takahashi, D. and Hagihara, K. (2003). Combinatorial effects of nonsteroidal anti-inflammatory drugs and food constituents on production of prostaglandin E2 and tumor necrosis factor alpha in RAW264.7 murine macrophages. Bio-sci. Biotechnol Biochem., 67, 1056–1062.
Nathan, C. and Xie, Q.W. (1994). Nitric oxide synthases: roles, tolls, and controls. Cell, 78, 915–918.
Numata, M., Suzuki, S., Miyazawa, N., Miyashita, A., Nagashima., Y., Inoue, S., Kaneko, T. and Okubo, T. (1998). Inhibition of inducible nitric oxide synthase prevents LPS-induced acute lung injury in dogs. J. Immunol, 160, 3031–3037.
Verma, N., Tripathi, S.K., Sahu, D., Das, H.R. and Das, R.H. (2010). Evaluation of inhibitory activities of plant extracts on production of LPS-stimulated pro-inflammatory mediators in J774 murine macrophages. Mol. Cell. Biochem., 336, 127–135.
Wu, L.C., Fan, N.C., Lin, M.H., Chu, I.R, Huang, S.J., Hu, C.Y. and Han, S.Y. (2008). Anti-inflammatory effect of spilanthol from Spilanthes acmella on murine macrophage by down-regulating LPS-induced inflammatory mediators. J. Agric. Food Chem., 56, 2341–2349.
Yoona, S., Yub, M., Pyunb, Y., Hwangb, J., Chuc, D., Juneja, L. and Mourao, P.A.S. (2003). The nontoxic mushroom Auricularia auricula contains a polysaccharide with anticoagulant activity mediated by antithrombin. Thrombosis Research, 112, 151–158.
Yuan, Z., He, P., Cui, J. and Takeuchi, H. (1998). Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricula-judae Quel, on genetically diabetic KK-Ay mice. Bioscience, Biotechnology, and Biochemistry, 62, 1898–1903.
Zhang, G. and Ghosh, S. (2000). Molecular mechanisms of NF-KB activation induced by bacterial lipopolysaccharide through Toll like receptors. J. Endotoxin. Res., 6, 453–457.
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Damte, D., Reza, M.A., Lee, SJ. et al. Anti-inflammatory Activity of Dichloromethane Extract of Auricularia auricula-judae in RAW264.7 Cells. Toxicol Res. 27, 11–14 (2011). https://doi.org/10.5487/TR.2011.27.1.011
- Auricularia auricula-judae
- Nitric oxide
- Inflammatory cytokines