Abstract
tert-Butyl acetate (TBAc) is an organic solvent, which is commonly used in architectural coatings and industrial solvents. It has recently been exempted from the definition of a volatile organic compound (VOC) by the Air Resources Board (ARB). Since the use of TBAc as a substitute for other VOCs has increased, thus its potential risk in humans has also increased. However, its inhalation toxicity data in the literature are very limited. Hence, inhalation exposure to TBAc was carried out to investigate its toxic effects in this study. Adult male rats were exposed to TBAc for 4 h for 1 day by using a nose-only inhalation exposure chamber (low dose, 2370 mg/m3 (500 ppm); high dose, 9482 mg/m3 (2000 ppm)). Sham-treated control rats were exposed to clean air in the inhalation chamber for the same period. The animals were killed at 2, 7, and 15 days after exposure. At each time point, body weight measurement, bronchoal-veolar lavage fluid (BALF) analysis, histopathological examination, and biochemical assay were performed. No treatment-related abnormal effects were observed in any group according to time course. Based on those findings, the median lethal concentration (LC50) of TBAc was over 9482 mg/m3 in this study. According to the MSDS, the 4 h LC50 for TBAc for rats is over 2230 mg/m3. We suggested that this value is changed and these findings may be applied in the risk assessment of TBAc which could be beneficial in a sub-acute study.
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References
American Conference of Governmental Industrial Hygienists (ACGIH). (1986). Documentation of the Threshold Limit Values and Biological Exposure Indices (5th edition), ACGIH, Cincinnati, OH, p. 74.
American Conference of Governmental Industrial Hygienists (ACGIH). (1991). tert-Butyl acetate in Documentation of the Threshold Limit Values and Biological Exposure Indices. ACGIH, Cincinnati, OH, p. 167.
Bennick, J.E. (1997). Acute inhalation toxicity study in rats. Study No. 3639-97, Stillmeadow Inc., Sugar Land, TX (available from the National Technical Information Service (NTIS), Springfield, VA; Order No. OTSO0573684-1).
Budroe, J.D., Brown,J.P., Salmon, A.G. and Marty M.A. (2004). Acute toxicity and cancer risk assessment values for tert-butyl acetate. Regul. Toxicol. Pharmacol, 40, 168–176.
DeGeorge, G. (1997). Single dose oral toxicity in rats/LD50 in rats. Research Project No. MB 97-6119.01, MB Research Laboratories Inc., Spinnerstown, PA (available from the National Technical Information Service (NTIS), Springfield, VA; Order No. OTSO0573684-1).
Giknis, M.L.A. and Clifford, C.B. (2006). Clinical laboratory parameters for Crl:CD(SD) rats, Charles River Laboratories, p. 10.
Girkin R., Kirkpatrick D. (2000). 14C-[tertwry-butyl-acetate] metabolism and pharmacokinetic study in the rat after inhalation. Report to Lyondell Chemicals Worldwide, Newtown Square, PA. Huntingdon Life Sciences Ltd., Huntingdon, Cambridgeshire, p. 1–90.
Groth, G. and Freundt, K.J. (1994). Inhaled tert-butyl acetate and its metabolite fert-butyl alcohol accumulate in the blood during exposure. Hum. Exp. Toxicol, 13, 478–480.
Hazardous Substances Data Bank (HSDB). (2007). National Library of Medicine, Bethesda, MD. Available at http://tox-net.nlm.nih.gov/cgi-bin/sis/htmlgen?HSDB.
Industrial Bio-Test Laboratories Inc. (1958). Toxicity studies on TFA-168. tert-Butyl acetate. Report to Albemarle Corporation, Baton Rouge, LA. Industrial Bio-Test Laboratories Inc., North-brook, IL. EPA/OTS Doc x 86940000229, NTIS/OTS0556824.
International Labour Organization (ILO) (1983). Encyclopedia of Occupational Health and Safety (Vols. I and II), Geneva, Switzerland, p. 783.
Kang, B.H., Kim, Y.B., Lee, H.S., Kim, Y.H., Im, W.J. and Ha, C.S. (2004). Background data on hematology, blood biochemistry and organ weights for 2 weeks and 4 weeks repeated-dose toxicity studies using Sprague-Dawley (SD) rats. Kor. J. Lab. Anim. Sci., 20, 134–140.
Kasai, T., Nishizawa, T., Arito, H., Nagano, K., Yamamoto, S., Matsushima, T. and Kawamoto, T. (2002). Acute and sub-chronic inhalation toxicity of chloroform in rats and mice. J. Occup. Health, 44, 193–202.
Kay, I.H. (1953). Toxicity studies on TFA-168. Report to The Texas Company. Industrial Bio-Test Laboratories Inc., North-brook, IL (available from the National Technical Information Service (NTIS), Springfield, VA; Order No. OTS00556824).
Kenney, T.J. (1999). tertiary-Butyl acetate acute (six-hour) inhalation study in rats. Report to Lyondell Chemicals Worldwide, Newtown Square, PA. Huntingdon Life Sciences Ltd., Huntingdon, Cambridgeshire, pp. 1–50.
Kenney, TJ. (2000). tertiary-Butyl acetate: 14 day repeat dose snout only inhalation toxicity range finding study in rats. Report to Lyondell Chemicals Worldwide, Newtown Square, PA. Huntingdon Life Sciences Ltd., Huntingdon, Cambridgeshire, pp. 1-107.
OECD (2009). Acute Inhalation Toxicity Testing. OECD guideline for testing of chemicals No. 403, OECD, Paris. Available at http://www.oecd.org/env/testguidelines.
Park, H.J., Yang, M.J., Oh, J.H., Yang, Y.S., Kwon, M.S., Song, C.W. and Yoon, S. (2010). Genome-wide transcriptional response during the development of bleomycin-induced pulmonary fibrosis in Sprague-Dawley rats. Toxicol Res., 26, 137–147.
Proctor, N.H., Hughes, J.P. and Fischman, M.L. (1988). Chemical Hazards of the Workplace (2nd edition), IB. Lippincott & Co., Philadelphia, PA, p. 105.
Pryhuber, G.S., Huyck, H.L., Baggs, R., Oberdorster, G. and Finkelstein, J.N. (2003). Induction of chemokines by low-dose intratracheal silica is reduced in TNFR I (p55) null mice. Toxicol Sci., 72, 150–157.
Rothenberg, S.J., Parker, R.M., York, R.G., Dearlove, G.E., Martin, M.M., Denny, K.H., Lief, S.D., Hoberman, A.M. and Christian, M.S. (2000). Lack of effects of nose-only inhalation exposure on testicular toxicity in male rats. Toxicol. Sci., 53, 127–134.
Song, K.S., Park, K.H., Kim, J.H., Han, D.U., Chae, C.H., Park, S.J., Kim, H.W., Kim, J.S., Park, J.H., Eu, G.J., Hua, J., Cho, H.S., Hwang, S.K., Chang, S.H., Yu, K.N., Kim, W.J., Kwon, J.T., Bhandari, D., Tehrani, A.M. and Cho, M.H. (2005). 90-day inhalation toxicity of dimethylamine in F344 rats. Toxicol Res., 21, 179–186.
Stillmeadow Inc. (1997). tert-Butyl acetate. Vol. V Acute inhalation toxicity study in rats. Sponsored by MB Research Laboratories Inc., Spinnerstown, PA, for Arco Chemical Company, Newtown Square, PA. Submitted to US Environmental Protection Agency (EPA), November 4, 1997. (EPA/Office of Technical Service (OTS), Doc. #87980000002) (available from the National Technical Information Service (NTIS), Springfield, VA; Order No. OTS0573684-1).
Yang, M.J., Yang, Y.S., Kim, Y.B., Cho, K.H., Heo, J.D., Lee, K. and Song, C.W. (2008). Noninvasive monitoring of bleomycin-induced lung injury in rats using pulmonary function test. Toxicol Res., 24, 273–280.
Yang, Y.S., Aim, T.H., Lee, J.C., Moon, C.J., Kim, S.H., Park, S.C., Chung, Y.H., Kim, H.Y and Kim, J.C. (2007). Effects of tert-butyl acetate on maternal toxicity and embryo-fetal development in Sprague-Dawley rats. Birth Defects Res. B Dev. Reprod. Toxicol, 80, 374–382.
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Yang, YS., Lee, J., Kwon, S. et al. LC50 Determination of tert-Butyl Acetate using a Nose Only Inhalation Exposure in Rats. Toxicol Res. 26, 293–300 (2010). https://doi.org/10.5487/TR.2010.26.4.293
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DOI: https://doi.org/10.5487/TR.2010.26.4.293