The hydrothermal vent clam Calyptogena magnifica (Bivalvia: Mollusca) is a member of the Vesicomyidae. Species within this family form symbioses with chemosynthetic Gammaproteobacteria. They exist in environments such as hydrothermal vents and cold seeps and have a rudimentary gut and feeding groove, indicating a large dependence on their endosymbionts for nutrition. The C. magnifica symbiont, Candidatus Ruthia magnifica, was the first intracellular sulfur-oxidizing endosymbiont to have its genome sequenced (Newton et al. 2007). Here we expand upon the original report and provide additional details complying with the emerging MIGS/MIMS standards. The complete genome exposed the genetic blueprint of the metabolic capabilities of the symbiont. Genes which were predicted to encode the proteins required for all the metabolic pathways typical of free-living chemoautotrophs were detected in the symbiont genome. These include major pathways including carbon fixation, sulfur oxidation, nitrogen assimilation, as well as amino acid and cofactor/vitamin biosynthesis. This genome sequence is invaluable in the study of these enigmatic associations and provides insights into the origin and evolution of autotrophic endosymbiosis.
Chemosynthetic symbioses, initially discovered at hydrothermal vents, also exist in shallow mud flats and seagrass beds, and deep sea cold methane seeps . In each case it is clear that these symbioses play major roles in community structuring and sulfur and carbon cycling. However, despite the widespread occurrence of these partnerships, little is known of the intricacies of host-symbiont interaction or symbiont metabolism due to their inaccessibility and our inability to culture either partner separately.
The giant clam, Calyptogena magnifica Boss and Turner (Bivalvia: Vesicomyidae), was one of the first organisms described after the discovery of hydrothermal vents. Vesicomyidae is a relatively old family, with fossil records and phylogenies dating them at 50–100 Ma . C. magnifica grows to a large size (>26 cm in length), despite having a reduced gut and ciliary food groove , presenting a conundrum regarding how it acquires sufficient nutrients. The discovery of chemoautotrophic, Gammaproteobacterial endosymbionts, now named Candidatus Ruthia magnifica (in memory of Prof. Ruth Turner), within C. magnifica gill bacteriocytes [4,5] helped to solve the mystery surrounding the nutrition of this clam. The host depends largely on these endosymbionts for its carbon, as indicated by its anatomy and by stable carbon isotopic ratios . However, how the host satisfies the rest of its nutritional requirements remained unknown.
Vesicomyid symbionts are presumed to be obligately symbiotic as they have a relatively reduced genome size [7–9], and are transmitted vertically between successive host generations via the egg . Evidence has been presented indicating a single Gammaproteobacterial symbiont is present in vesicomyids that have been examined via rRNA phylotyping . However recent evidence suggests that vesicomyids may harbor two symbiont phylotypes, both of which fall into the same clade but are distinct phylotypes. Thus the clams may acquire divergent symbionts laterally via uptake from an environmental population or horizontal transfer from co-occurring hosts .
Here we present a classification and a set of features (Figure 1, Figure 2, Table 1) for Candidatus R. magnifica, together with a description of the complete genome sequence and annotation originally presented in .
Candidatus Ruthia magnifica is the chemosynthetic gill endosymbiont of the giant clam, Calyptogena magnifica Boss and Turner (Bivalvia: Vesicomyidae) (Figure 1). Vesicomyid clams are conspicuous fauna at many deep-sea hydrothermal-vent and cold-seep habitats. Candidatus R. magnifica, a member of the phylum Gammaproteobacteria, falls within the vesicomyid symbiont clade which is a sister group to vent and seep mussel chemosynthetic symbionts of the subfamily Bathymolidinae (Figure 2).
The Calyptogena magnifica symbiont Candidatus Ruthia magnifica was selected for sequencing because this symbiosis is one of the dominant macrofauna at vent sites in the eastern Pacific Ocean. Knowledge of the metabolic capabilities of this symbiosis provides new perspectives on the coupling of carbon and sulfur fluxes in the deep-sea, a substantial reservoir in the global carbon cycle. In addition, this genome provides insights into the origin and evolution of autotrophic endosymbiosis. This project was funded by a US Department of Energy as part of the Joint Genome Institute Community Sequencing Program.
The complete genome sequence was finished in January 2006 and originally described in Newton et al. 2007 . The GenBank accession number for the symbiont genome is CP000488.1 and is listed in the Genomes OnLine Database (GOLD) as project Gc00468. A summary of the project information is shown in Table 2.
Specimen collection and DNA extraction
Calyptogena magnifica clams were collected using DSV Alvin at the East Pacific Rise, 9°N vent field, during a cruise on the R/V Atlantis in December 2004. Symbiont containing gills were dissected out of the clams, frozen in liquid nitrogen, and kept at −80°C until processed in the lab. Gill tissues were ground in liquid nitrogen, placed in lysis buffer (20 mM EDTA, 10 mM Tris-HCl, pH 7.9, 0.5 mg/ml lysozyme, 1% Triton X-100, 200 mM NaCl, 500 mM guanidine-HCl,) and incubated at 40°C for 2 hr. After subsequent RNase (20 µg/ml, 37°C, 30 min) and proteinase K (20 µg/ml, 50°C, 1.5 hr) treatments, the samples were centrifuged and the supernatant was transferred onto Qiagen Genomic Tip columns and processed according to manufacturer’s protocol (QIAGEN, Valencia, CA).
Genome sequencing and assembly
The genome was sequenced by Sanger sequencing of 3kb, 8kb and fosmid libraries. All general aspects of construction and sequencing performed at the JGI can be found on the JGI website.
Briefly, 22.15 Mb of phred Q20 sequence were generated: 9.43 Mb from 13,755 reads from the small insert pUC library, 8.79 Mb from 13,824 reads from the medium insert pMCL library, and 3.93 Mb from 9,216 reads from the fosmid library. The DNA sequences derived from the Candidatus Ruthia magnifica libraries were estimated to be 20% contaminated with the Calyptogena magnifica host genome. Although this level of contamination could confound finishing efforts, the bacterial genome was readily identifiable in this study. The 36,795 sequencing reads were blasted against a database containing all mollusk sequences available in Genbank and the 4× draft gastropod Lottia gigantea genome sequence available at the JGI. A total of 498 reads were removed based on hits to this mollusk database.
The remaining 24,595 reads were base called, vector trimmed, and assembled using parallel phrap. One large, bacterial scaffold containing the Candidatus R. magnifica 16S rRNA gene resulted. The R. magnifica scaffold consisted of only 2 contigs spanned by 33 fosmid clones, contained 17,307 reads, 1,156,121 consensus bp, was covered by an average read depth of 14×, and had a G+C content of 34%. The next largest scaffold was only 29 kb long, with an average read depth of ∼7× and an average G+C content of 55%. BLASTn indicated that this latter scaffold encoded ribosomal genes closely related to those of Caenorhabditis briggsae and its binning (based on GC content and read depth) with a small scaffold containing the C. magnifica 18S rRNA gene confirmed its eukaryotic host origin.
The DNA sequence was submitted to the TIGR auto-annotation pipeline (currently hosted at JCVI). Included in the pipeline is gene finding with Glimmer , Blast-extend-repraze (BER) searches, HMM searches, TMHMM searches, SignalP predictions, and automatic annotations from AutoAnnotate. The output from the TIGR Annotation Service was transferred to a MySQL database. Additional gene prediction analysis and manual functional annotation was performed using Manatee (http://manatee.sourceforge.net) .
Metabolic network analysis
The metabolic Pathway/Genome Database (PGDB) was computationally generated by the Pathologic program using Pathway Tools software version 14.0  and MetaCyc version 13.1 , based on annotated EC numbers and a customized enzyme name mapping file. The PGDB has not been subjected to manual curation and may contain errors.
The genome consists of one circular chromosome with 1,160,782 bp (Figure 3). For the complete genome, 1,118 genes were predicted, 1076 of which are protein-coding genes. 837 of the protein coding genes were assigned to a putative function with the remaining annotated as hypothetical proteins. The properties and the statistics of the genome are summarized in Table 3. The distribution of genes into COG functional categories is presented in Table 4. A cellular overview diagram is presented in Figure 4, followed by a summary of metabolic network statistics shown in Table 5.
Insights from the genome sequence
The Candidatus R. magnifica genome has revealed striking differences between the chemosynthetic endosymbiont genomes and those of other obligate mutualistic symbionts for which genomic data are available. The genome is small (1.1 Mb) and has a low G+C content (34%) compared to free-living sulfur oxidizing proteobacteria . These common features of endosymbionts are likely the result of genome reduction and accumulation of point mutations that occur over evolutionary time across diverse symbiont species . This trend has been observed in recently evolved symbioses such the insect endosymbionts (30–250 Ma) , as well as in chloroplasts (∼1,800-2,100 Ma) .
However, Candidatus R. magnifica stands out in that its genome is relatively large for a maternally transmitted endosymbiont. For example, the genomes of the Gammaproteobacterial Buchnera which are endosymbionts of aphids, are ∼85% smaller than closely related free-living species like E. coli. In contrast, the genome of Candidatus R. magnifica is ∼24% the size of E. coli K12 and ∼55% smaller than Thiomicrospira crunogena, a free-living, Gammaproteobacterial, sulfur-oxidizing chemoautotroph isolated from vents .
The genome lacks any form of mobile DNA content. Neither transposon- nor phage-related sequences were identified except for the putative prophage repressor gene LexA (EC 126.96.36.199).
The genome encodes enzymes specific for carbon fixation via the Calvin cycle; including a form II ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBisCO, EC 188.8.131.52) and phosphoribulokinase (EC 184.108.40.206) . Energy for carbon fixation appears to be derived from sulfur oxidation via the “sulfur oxidation (sox) pathway” and dsr (dissimilatory sulfite reductase) pathway .
Remarkably, the genome lacks the Calvin cycle homologs sedoheptulose 1,7-bis-phosphatase (SBPase, EC 220.127.116.11) and fructose 1,6-bis-phosphatase (FBPase, EC 18.104.22.168), suggesting that the regeneration of ribulose 1,5-bisphosphate may not follow conventional pathways . Instead, the genome contains a reversible pyrophosphate-dependent phosphofructokinase (EC 22.214.171.124) homolog that may be used to generate fructose 6-phosphate .
The central intermediary metabolism of Candidatus R. magnifica produces all the intermediates necessary for the synthesis of amino acids, nucleotides, fatty acids, vitamins and cofactors, which are thought to be supplied to the host . Notably, the symbiont lacks homologs of fumarate reductase, succinate dehydrogenase, and succinyl-coA synthase. However, the genome encodes isocitrate lyase, part of the glyoxylate shunt, suggesting succinate production from isocitrate .
Although able to synthesize 10 vitamins/cofactors, the cobalamin (B12) biosynthesis pathway is conspicuously absent . Since cobalamin is a cofactor for methionine synthase  and since Candidatus R. magnifica encodes a cobalamin-independent methionine synthase, the host might not require cobalamin.
Several transporters involved in chemoautotrophy (sulfate exporters), nitrogen assimilation (nitrate and ammonium transporters), inorganic compounds (TrkAH, MgtE family, CaCA family and PiT family), and heavy metals (ZnuABC, RND superfamily, iron permeases) were identified .
The diverse metabolic capabilities of Candidatus R. magnifica, inferred from the genome sequence, confirm and extend our understanding of host nutritional dependency.
Cavanaugh CM, McKiness ZP, Newton ILG, Stewart FJ. (2006). Marine chemosynthetic symbioses. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH and Stackebrandt E (eds). The Prokaryotes. Third Edition. A Handbook on the Biology of Bacteria. Springer: New York. pp 475–507.
Peek A, Gustafson R, Lutz R, Vrijenhoek R. Evolutionary relationships of deep-sea hydrothermal vent and cold-water seep clams (Bivalvia: Vesicomyidae): Results from the mitochondrial cytochrome oxidase subunit I. Mar Biol 1997; 130:151–161. doi:10.1007/s002270050234
Boss KJ, Turner RD. The giant white clam from the Galapagos Rift, Calyptogena magnifica n. sp. (Bivalvia; Vesicomyidae). Malacologia 1980; 20:161–194.
Cavanaugh CM. Symbiotic Chemoautotrophic Bacteria in Marine-Invertebrates from Sulfide-Rich Habitats. Nature 1983; 302:58–61. doi:10.1038/302058a0
Felbeck H, Somero GN. Primary Production in Deep-Sea Hydrothermal Vent Organisms — Roles of Sulfide-Oxidizing Bacteria. Trends Biochem Sci 1982; 7:201–204. doi:10.1016/0968-0004(82)90088-3
Fisher CR, Childress JJ, Arp AJ, Brooks JM, Distel DL, Dugan JA, Felbeck H, Fritz LW, Hessler RR, Johnson KS, et al. Variations in the hydrothermalvent clam Calyptogena magnifica at the Rose Garden vent on the Galapagos Spreading Center. Deep-Sea Res 1988; 35:1811–1831. doi:10.1016/0198-0149(88)90051-9
Kuwahara H, Yoshida T, Takaki Y, Shimamura S, Nishi S, Harada M, Matsuyama K, Takishita K, Kawato M, Uematsu K, et al. Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea clam, Calyptogena okutanii. Curr Biol 2007; 17:881–886. PubMed doi:10.1016/j.cub.2007.04.039
Newton IL, Girguis PR, Cavanaugh CM. Comparative genomics of vesicomyid clam (Bivalvia: Mollusca) chemosynthetic symbionts. BMC Genomics 2008; 9:585. PubMed doi:10.1186/1471-2164-9-585
Newton IL, Woyke T, Auchtung TA, Dilly GF, Dutton RJ, Fisher MC, Fontanez KM, Lau E, Stewart FJ, Richardson PM, et al. The Calyptogena magnifica chemoautotrophic symbiont genome. Science 2007; 315:998–1000. PubMed doi:10.1126/science.1138438
Hurtado LA, Mateos M, Lutz RA, Vrijenhoek RC. Coupling of bacterial endosymbiont and host mitochondrial genomes in the hydrothermal vent clam Calyptogena magnifica. Appl Environ Microbiol 2003; 69:2058–2064. PubMed doi:10.1128/AEM.69.4.2058-2064.2003
Peek AS, Feldman RA, Lutz RA, Vrijenhoek RC. Cospeciation of chemoautotrophic bacteria and deep sea clams. Proc Natl Acad Sci USA 1998; 95:9962–9966. PubMed doi:10.1073/pnas.95.17.9962
Stewart FJ, Young CR, Cavanaugh CM. Lateral symbiont acquisition in a maternally transmitted chemosynthetic clam endosymbiosis. Mol Biol Evol 2008; 25:673–687. PubMed doi:10.1093/molbev/msn010
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000; 25:25–29. PubMed doi:10.1038/75556
Delcher AL, Harmon D, Kasif S, White O, Salzberg SL. Improved microbial gene identification with GLIMMER. Nucleic Acids Res 1999; 27:4636–4641. PubMed doi:10.1093/nar/27.23.4636
Karp PD, Paley S, Romero P. The Pathway Tools software. Bioinformatics 2002; 18(Suppl 1):S225–S232. PubMed
Caspi R, Foerster H, Fulcher CA, Kaipa P, Krummenacker M, Latendresse M, Paley S, Rhee SY, Shearer AG, Tissier C, et al. The MetaCyc Database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases. Nucleic Acids Res 2007; 36:D623–D631. PubMed doi:10.1093/nar/gkm900
Wernegreen JJ. For better or worse: genomic consequences of intracellular mutualism and parasitism. Curr Opin Genet Dev 2005; 15:572–583. PubMed doi:10.1016/j.gde.2005.09.013
Gil R, Sabater-Munoz B, Latorre A, Silva FJ, Moya A. Extreme genome reduction in Buchnera spp.: toward the minimal genome needed for symbiotic life. Proc Natl Acad Sci USA 2002; 99:4454–4458. PubMed doi:10.1073/pnas.062067299
Martin W, Stoebe B, Goremykin V, Hapsmann S, Hasegawa M, Kowallik KV. Gene transfer to the nucleus and the evolution of chloroplasts. Nature 1998; 393:162–165. PubMed doi:10.1038/30234
Scott KM, Sievert SM, Abril FN, Ball LA, Barrett CJ, Blake RA, Boller AJ, Chain PS, Clark JA, Davis CR, et al. The genome of deep-sea vent chemolithoautotroph Thiomicrospira crunogena XCL-2. PLoS Biol 2006; 4:e383. PubMed doi:10.1371/journal.pbio.0040383
Kemp RG, Tripathi RL. Pyrophosphate-dependent phosphofructo-1-kinase complements fructose 1,6-bisphosphatase but not phosphofructokinase deficiency in Escherichia coli. J Bacteriol 1993; 175:5723–5724. PubMed
Vanni P, Giachetti E, Pinzauti G, McFadden BA. Comparative structure, function and regulation of isocitrate lyase, an important assimilatory enzyme. Comp Biochem Physiol B 1990; 95:431–458. PubMed doi:10.1016/0305-0491(90)90002-B
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD. EcoCyc: a comprehensive database resource for Escherichia coli. Nucleic Acids Res 2004; 33:D334–D337. doi:10.1093/nar/gki108
This research was funded by a grant from the Office of Science of the U.S. Department of Energy to CMC and JAE, a Howard Hughes Medical Institute Predoctoral Fellowship to ILGN and a Rubicon grant from the Netherlands Organisation for Scientific Research (NWO) to GR. The work was conducted in part at the U.S. Department of Energy Joint Genome Institute, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. We thank Eddy Rubin and David Bruce for project management.
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Roeselers, G., Newton, I.L.G., Woyke, T. et al. Complete genome sequence of Candidatus Ruthia magnifica. Stand in Genomic Sci 3, 163–173 (2010). https://doi.org/10.4056/sigs.1103048
- Hydrothermal vent