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RGD peptide-displaying M13 bacteriophage/PLGA nanofibers as cell-adhesive matrices for smooth muscle cells

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Abstract

Extracellular matrices (ECMs) are network structures that play an essential role in regulating cellular growth and differentiation. In this study, novel nanofibrous matrices were fabricated by electrospinning M13 bacteriophage and poly(lactic-co-glycolic acid) (PLGA) and were shown to be structurally and functionally similar to natural ECMs. A genetically-engineered M13 bacteriophage was constructed to display Arg-Gly-Asp (RGD) peptides on its surface. The physicochemical properties of RGD peptide-displaying M13 bacteriophage (RGD-M13 phage)/PLGA nanofibers were characterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. We used immunofluorescence staining to confirm that M13 bacteriophages were homogenously distributed in RGD-M13 phage/PLGA matrices. Furthermore, RGD-M13 phage/PLGA nanofibrous matrices, having excellent biocompatibility, can enhance the behaviors of vascular smooth muscle cells. This result suggests that RGD-M13 phage/PLGA nanofibrous matrices have potentials to serve as tissue engineering scaffolds.

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Authors and Affiliations

  1. Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Korea

    Yong Cheol Shin, Jong Ho Lee, Oh Seong Jin, Eun Ji Lee, Lin Hua Jin, Chang-Seok Kim, Suck Won Hong & Dong-Wook Han

  2. Department of Nanomaterials Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 609-735, Korea

    Chuntae Kim & Jin-Woo Oh

Authors
  1. Yong Cheol Shin
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  2. Jong Ho Lee
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  3. Oh Seong Jin
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  4. Eun Ji Lee
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  5. Lin Hua Jin
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  6. Chang-Seok Kim
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  7. Suck Won Hong
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  8. Dong-Wook Han
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  9. Chuntae Kim
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  10. Jin-Woo Oh
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Correspondence to Dong-Wook Han.

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Shin, Y.C., Lee, J.H., Jin, O.S. et al. RGD peptide-displaying M13 bacteriophage/PLGA nanofibers as cell-adhesive matrices for smooth muscle cells. Journal of the Korean Physical Society 66, 12–16 (2015). https://doi.org/10.3938/jkps.66.12

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