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MTR and In-vivo 1H-MRS studies on mouse brain with parkinson’s disease

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Abstract

The aim of this study was to investigate whether the changes in the magnetization transfer ratio (MTR) histogram are related to specific characteristics of Parkinson’s disease (PD) and to investigate whether the MTR histogram parameters are associated with neurochemical dysfunction by performing in vivo proton magnetic resonance spectroscopy (1H-MRS). MTR and in vivo 1H-MRS studies were performed on control mice (n = 10) and 1-methyl-1,2,3,6-tetrahydropyridine intoxicated mice (n = 10). All the MTR and in vivo 1H-MRS experiments were performed on a 9.4 T MRI/MRS system (Bruker Biospin, Germany) using a standard head coil. The protondensity fast spin echo (FSE) images and the T2-weighted spin echo (SE) images were acquired with no gap. Outer volume suppression (OVS), combined with the ultra-short echo-time stimulated echo acquisition mode (STEAM), was used for the localized in-vivo 1H-MRS. The quantitative analysis of metabolites was performed from the 1H spectra obtained in vivo on the striatum (ST) by using jMRUI (Lyon, France). The peak height of the MTR histograms in the PD model group was significantly lower than that in the control group (p < 0.05). The midbrain MTR values for volume were lower in the PD group than the control group(p < 0.05). The complex peak (Glx: glutamine+glutamate+ GABA)/creatine (Cr) ratio of the right ST in the PD group was significantly increased as compared to that of the control group. The present study revealed that the peak height of the MTR histogram was significantly decreased in the ST and substantia nigra, and a significant increase in the Gl x /Cr ratio was found in the ST of the PD group, as compared with that of the control group. These findings could reflect the early phase of neuronal dysfunction of neurotransmitters.

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Authors and Affiliations

  1. Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea

    Moon-Hyun Yoon & Bo-Young Choe

  2. Department of Radiology, Seoul National University Hospital, Seoul, 110-744, Korea

    Hyeon-Jin Kim

  3. Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon, 406-799, Korea

    Jin-Yeung Chung

  4. Acupuncture & Meridian Science Research Center, Kyung Hee University, Seoul, 130-701, Korea

    Ah-Reum Doo, Hi-Joon Park & Seung-Nam Kim

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  1. Moon-Hyun Yoon
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  2. Hyeon-Jin Kim
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Yoon, MH., Kim, HJ., Chung, JY. et al. MTR and In-vivo 1H-MRS studies on mouse brain with parkinson’s disease. Journal of the Korean Physical Society 61, 1852–1859 (2012). https://doi.org/10.3938/jkps.61.1852

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  • DOI: https://doi.org/10.3938/jkps.61.1852

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