Screening and compound isolation from natural plants for anti-allergic activity

Abstract

Drug discovery for the treatment of allergic disease is an important field in human health. Natural plants and medicinal herbs used commercially in Korea were screened for degranulation inhibitory activities of 164 plant species in the RBL-2H3 cell line, and their activities were investigated using the β-hexosaminidase release assay; 34 species were active. Lactuca indica var. laciniata had the highest degranulation inhibitory activity, and Plantago asiatica, Cimicifuga heracleifolia, Poncirus trifoliate, Lycopus ramosissimus, Quercus mongolica, Anemarrhena asphodeloides, Eugenia caryophyllata, and Curcuma longa exhibited degranulation inhibitory capacity higher than 50% at 125 μg/mL. One compound was isolated by activity-guided fractionation and isolation from methanol (MeOH) extract of A. asphodeloides, which has not yet been identified as constituent for anti-allergic and atopic effects. The structure of isolated compound was elucidated on the basis of NMR and Mass spectrometric data as nyasol, which was highly effective at very low dose (70.98±1.57% at 50 μg/mL). These results demonstrate that natural products and contained compounds may be useful in the therapy of allergic diseases, such as atopic dermatitis and asthma.

This is a preview of subscription content, access via your institution.

References

  1. Bae G, Yu JR, Lee J, Chang J, and Seo EK (2007) Identification of the nyasol and structurally related compounds as the active principles from Anemarrhena asphodeloides against repiratory syncytial virus (RSV). Chem Biodiver 4, 2231–2235.

    Article  CAS  Google Scholar 

  2. Chainani-Wu N (2003) Safety and anti-inflammatory activity of curcumin: A component of tumeric (Curcuma longa). J Altern Complement Med 9, 161–168.

    Article  Google Scholar 

  3. Choi OH, Kim JH, and Kinet JP (1996) Calcium mobilization via sphingosine kinase in signalling by the Fc epsilon RI antigen receptor. Nature 380, 634–636.

    Article  CAS  Google Scholar 

  4. Choo MK, Park EK, Han MJ, and Kim DH (2003) Antiallergic activity of ginseng and its ginsenosides. Planta Med 69, 518–522.

    Article  CAS  Google Scholar 

  5. Fischer MJ, Paulussen JJ, Horbach DA, Roelofsen EP, van Miltenburg JC, de Mol NJ, and Janssen LH (1995) Inhibition of mediator release in RBL-2H3 cells by some H1-antagonist derived anti-allergic drugs: Relation to lipophilicity and membrane effects. Inflamm Res 44, 92–97.

    Article  CAS  Google Scholar 

  6. Jeong SJ, Ahn NH, and Kim YC (1999) Norlignans with hyaluronidase inhibitory activity from Anemarrhena asphodeloides. Planta Med 65, 367–368.

    Article  CAS  Google Scholar 

  7. Kim SH and Shin TY (2006) Effect of Dracocephalum argunense on mast-cell-mediated hypersensitivity. Int Arch Allergy Immunol 139, 87–95.

    Article  Google Scholar 

  8. Kim SH, Choi CH, Kim SY, Eun JS, and Shin TY (2004) Antiallergic effects of Artemisia iwayomogi on mast cell-mediated allergy model. Exp Biol Med 230, 82–88.

    Google Scholar 

  9. Kim SH, Kim SH, Kim SH, Moon JY, Park WH, Kim CH, and Shin TY (2006) Action of Dracocephalum argunense on mast cell-mediated allergy model. Biol Pharm Bull 29, 494–498.

    Article  CAS  Google Scholar 

  10. Lee HT and Ryu JH (1999) Hinokirecinol: A novel inhibitor of LTB4 binding to the human neutrophils. Planta Med 65, 391.

    Article  CAS  Google Scholar 

  11. Lim H, Nam JW, Seo EK, Kim YS, and Kim HP (2009) (-)-Nyasol (cis-hinokiresinol), a Norneolignan from the rhizomes of Anemarrhena asphodeloides, is a broad spectrum inhibitor of eicosanoid and nitric oxide production. Arch Pharm Res 32, 509–1514.

    Google Scholar 

  12. Marquardt DL and Wasserman SI (1983) Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol 131, 934–939.

    CAS  Google Scholar 

  13. Marone G, Genovese A, Granata F, Forte V, Detoraki A, de Paulis A, and Triggiani M (2002) Pharmacological modulation of human mast cells and basophils. Clin Exp Allergy 32, 1682–1689.

    Article  CAS  Google Scholar 

  14. Murai M, Tamayama Y, and Nishibe S (1995) Phenylethanoids in the herb of Plantago lanceolata and inhibitory effect on arachidonic acid-induced mouse ear edema. Planta Med 61, 479–480.

    Article  CAS  Google Scholar 

  15. Park EK, Choo MK, Kim EJ, Han MJ, and Kim DH (2003) Antiallergic activity of ginsenoside Rh2. Biol Pharm Bull 26, 1581–1584.

    Article  CAS  Google Scholar 

  16. Schwartz LB, Austen KF, and Wasserman SI (1979) Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol 123, 1445–1450.

    CAS  Google Scholar 

  17. Shin TY, Kim SH, Suk K, Ha JH, Kim I, Lee MG, Jun CD, Kim SY, Lim JP, Eun JS, Shin HY, and Kim HM (2005) Anti-allergic effects of Lycopus lucidus on mast cell-mediated allergy model. Toxicol Appl Pharmacol 209, 255–262.

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding authors

Correspondence to In Su Kim or Se Chan Kang.

Additional information

J. P. Bak contributed equally.

J. B. Kim contributed equally.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bak, J.P., Kim, J.B., Park, J.H. et al. Screening and compound isolation from natural plants for anti-allergic activity. J. Korean Soc. Appl. Biol. Chem. 54, 367–375 (2011). https://doi.org/10.3839/jksabc.2011.058

Download citation

Key words

  • allergy
  • degranulation
  • medicinal herbs
  • RBL-2H3