Abstract
A multiplex real-time polymerase chain reaction (PCR) kit was developed for the qualitative detection of the Roundup-Ready Soybean (RRS). This system consists of a screening assay for cauliflower mosaic virus 35S promoter and nos terminator, and an event-specific assay for RRS containing the lectin gene as an internal positive control. The limit of detection value was determined to be approximately 0.1%.
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Kim, JH., Kim, JH., Baek, MA. et al. Multiplex real-time PCR kit for qualitative detection of genetically modified roundup ready soybean. J. Korean Soc. Appl. Biol. Chem. 54, 145–148 (2011). https://doi.org/10.3839/jksabc.2011.023
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DOI: https://doi.org/10.3839/jksabc.2011.023