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Cloning of tunicamycin biosynthetic gene cluster from Streptomyces chartreusis NRRL 3882

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Abstract

The putative tunicamycin biosynthetic gene cluster was identified in Streptomyces clavuligerus with genome mining approach and this genetic information was used to isolate the tunicamycin biosynthetic gene set from S. chartreusis. Gene inactivation of tun1 Sch abolished tunicamycin accumulation in S. chartreusis, confirming that the tunicamycin biosynthetic gene cluster was cloned.

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Authors and Affiliations

  1. Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, 449-728, Yongin, Republic of Korea

    Suman Karki, So-Yeon Kwon & Hyung-Jin Kwon

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  1. Suman Karki
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  2. So-Yeon Kwon
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  3. Hyung-Jin Kwon
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Correspondence to Hyung-Jin Kwon.

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Karki, S., Kwon, SY. & Kwon, HJ. Cloning of tunicamycin biosynthetic gene cluster from Streptomyces chartreusis NRRL 3882. J. Korean Soc. Appl. Biol. Chem. 54, 136–140 (2011). https://doi.org/10.3839/jksabc.2011.021

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  • DOI: https://doi.org/10.3839/jksabc.2011.021

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