Abstract
Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30°C for 3.5 days.
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Lee, D., Boo, KH., Woo, JK. et al. Production conditions for bruceanol b, an antibiotic that generates oxygen radical. J. Korean Soc. Appl. Biol. Chem. 54, 128–131 (2011). https://doi.org/10.3839/jksabc.2011.019
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DOI: https://doi.org/10.3839/jksabc.2011.019