In Vitro investigation on antioxidative effect of Inonotus obliquus extracts against oxidative stress on PC12 cells

Abstract

Antioxidative activities of various enzymatic extracts from Inonotus obliquus were evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical-scavenging activities using electron spin resonance spectrometer. The mushrooms were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (α-chymotrypsin, Alcalase, Flavourzyme, Neutrase, papain, pepsin, Protamex, and trypsin). Celluclast and Protamex extracts showed the highest DPPH radical-scavenging activities, and IC50 values were 0.155 and 0.151 mg/mL, respectively. All enzymatic extracts of I. obliquus had scavenging potencies to hydroxyl radical, and IC50 value of the pepsin extract with the highest activity was 0.847 mg/mL. Maltogenase and trypsin extracts showed the highest scavenging activities on alkyl radical, and IC50 values were 0.082 and 0.094 mg/mL, respectively. The pepsin extracts showed protective effect on H2O2-induced DNA damage. In addition, the pepsin extracts decreased generation of reactive oxygen species and cell death in PC-12 cells against H2O2-induced oxidative damage. The findings of the present study suggest that the enzymatic extracts of I. obliquus have antioxidative activity against oxidative stress on PC-12 cells.

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Correspondence to Pyo-Jam Park.

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Both authors contributed equally to this work.

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Kim, EK., Lee, SJ., Hwang, JW. et al. In Vitro investigation on antioxidative effect of Inonotus obliquus extracts against oxidative stress on PC12 cells. J. Korean Soc. Appl. Biol. Chem. 54, 112–117 (2011). https://doi.org/10.3839/jksabc.2011.016

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Key words

  • antioxidative activity
  • enzymatic hydrolysates
  • free radical
  • Innotus obliquus
  • neuroprotective
  • effect