Abstract
Anticancer effects of the mussel (Mytilus coruscus) cultivated in Korea were determined. Lipid extracts of Mytilus coruscus were prepared using extractions in several organic solvents: methanol, chloroform, hexane, methanol:chloroform=1:1, and chloroform:hexane=1:1. Anticancer activities of the extracts were evaluated using apoptosis assays analyzed by flow cytometry. The hexane extract exhibited the highest anticancer activity. This extract was further separated and purified using thin layer chromatography, through which lipid compounds were isolated, and their components were analyzed; the major fatty acids were eiocosadienoic acid (EA, C20:2), eicosapentaenoic acid (EPA, C20:5), and docosahexaenoic acid (DHA, C22:6), and these components were higher than those of the mussels cultivated in New Zealand and Italy. The partially purified lipid compounds exhibited an anticancer effect in several cancer cell lines. These results indicate that lipid extract of M. coruscus effectively inhibits in vitro tumor growth by inducing apoptosis of cancer cells.
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References
Baylin A, Kabagambe EK, Siles X, and Campos H (2002) Adipose tissue biomarkers of fatty acid intake. Am J Clin Nutr 76, 750–757.
Begin ME, Ells G, and Horrobin DF (1988) Polyunsaturated fatty acid induced cytotoxicity against tumor cells and its relationship to lipid peroxidation. J Natl Cancer Inst 80, 188–194.
Black PN, Faergeman NJ, and DiRusso CC (2000) Long-chain acyl-CoAdependent regulation of gene expression in bacteria, yeast and mammals. J Nutr 130, 305S-309S.
Byrd JC, Shinn C, Waselenko JK, Fuchs EJ, Lehman TA, Nguyen PL, Flinn IW, Diehl LF, Sausville E, and Grever MR (1998) Flavopiridol induces apoptosis in chronic lymphocytic leukemia cells via activation of caspase-3 without evidence of bcl-2 modulation or dependence on functional p53. Blood 92, 3804–3816.
Cesaretti M, Luppi E, Maccari F, and Volpi N (2004) Isolation and characterization of a heparin with high anticoagulant activity from the clam Tapes phylippinarum: Evidence for the presence of a high content of antithrombin III binding site. Glycobiology 14, 1275–1284.
Esterbauer H, Schaur RJ, and Zollner H (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 11, 81–128.
Hannun YA (1997) Apoptosis and the dilemma of cancer chemotherapy. Blood 89, 1845–1853.
Karmali RA, Marsh J, and Fuchs C (1984) Effect of omega-3 fatty acids on growth of a rat mammary tumor. J Natl Cancer Inst 73, 457–461.
Korostetski EI, Boroda AV, and Odintsova NA (2008) Changes in the lipid composition of embryonic cells of the mussel Mytilus trossulus during cryopreservation. Biofizika 53, 658–665.
Li PP and Ding XL (2006) The manufacture and nutritional analysis of the functional natural sauce from the decoction of Mytilus edulis. China Condiment 2, 17–19.
McPhee S, Hodges LD, Wright PFA, Wynne PM, Kalafatis N, Harney DW, and Macrides TA (2007) Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus. Comp Biochem Physiol B 146, 346–356.
Miller TE, Dodd J, Ormrod DJ, and Geddes R (1993) Anti-inflammatory activity of glycogen extracted from Perna canaliculus (NZ green-lipped mussel). Agents Actions 38, 139–142.
Morrison WR and Smith LM (1964) Preparation of fatty acid methylesters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 5, 600–608.
Murphy KJ, Mooney BD, Mann NJ, Nichols PD, and Sinclair AJ (2002) Lipid, FA, and sterol composition of New Zealand green lipped mussel (Perna canaliculus) and Tasmanian blue mussel (Mytilus edulis). Lipid 37, 587–595.
Neuhouser ML, Barnett MJ, Kristal AR, Ambrosone CB, King I, Thornquist M, and Goodman G (2007) (n−6) PUFA increase and dairy foods decrease prostate cancer risk in heavy smokers. J Nutr 137, 1821–1827.
Ovodova RG, Glazkova VE, Mikheyskaya LV, Molchanova VI, Isakov VV, Ovodov YS, and Molina LEF (1992) The structure of mytilan, a bioglycanimmunomodulator isolated from the mussel Crenomytilus grayanus. Carbohydr Res 223, 221–226.
Pegorier JP, May CL, and Girard J (2004) Control of gene expression by fatty acids. J Nutr 134, 2444S-2449S.
Pirini M, Manuzzi MP, Pagliarani A, Trombetti F, Borgatti AR, and Ventrella V (2007) Changes in fatty acid composition of Mytilus galloprovincialis (Lmk) fed on microalgal and wheat germ diets. Comp Biochem Physiol 147, 616–626.
Stefanov K, Seizova K, Brechany EY, and Christie WW (1992) An unusual fatty acid composition for a fresh-water mussel, Unio tumidus, from Bulgaria. J Nat Prod 55, 979–981.
Terry PD, Rohan TE, and Wolk A (2003) Intakes of fish and marine fatty acids and the risks of cancers of the breast and prostate and of other hormone-related cancers: a review of the epidemiologic evidence. Am J Clin Nutr 77, 532–543.
Ventrella V, Pirini M, Pagliarani A, Trombetti F, Manuzzi MP, and Borgatti AR (2008) Effect of temporal and geographical factors on fatty acid composition of M. galloprovincialis from the Adriatic sea. Comp Biochem Physiol B 149, 241–250.
Vock C, Gleissner M, Klapper M, and Döring F (2007) Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis. Biochim Biophys Acta 1770, 1283–1288.
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Kim, EK., Kim, YS., Lee, SJ. et al. Effect of partially purified lipid from the mussel Mytilus coruscus on apoptosis in cancer cells. J. Korean Soc. Appl. Biol. Chem. 54, 59–65 (2011). https://doi.org/10.3839/jksabc.2011.008
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DOI: https://doi.org/10.3839/jksabc.2011.008