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Determination of arsenic species in polished rice using a methanol-water digestion method


Inorganic arsenic (iAs) in food is much more toxic than organic. Four arsenic species, arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), and dimethylarsenic acid (DMA) in polished rice cultivated at the mining areas of Korea were evaluated. A methanol-water (1:1) mixture was used for extraction of As compounds and an anion Hamilton PRP X-100 column for As species separation in HPLC-ICP-MS. The limits of detection were 5.3, 3.7, 19.2, and 3.7 ώg/kg on dry weight basis for AsIII, AsV, MMA, and DMA, respectively. When this method was applied to eleven polished rice samples, the mean value of iAs content in total As was approximately 57%.

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Correspondence to Min-Kyoung Paik.

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Paik, MK., Kim, MJ., Kim, WI. et al. Determination of arsenic species in polished rice using a methanol-water digestion method. J. Korean Soc. Appl. Biol. Chem. 53, 634–638 (2010).

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Key words

  • arsenic
  • arsenic species
  • methanol-water digestion
  • rice