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Cytotoxicity of Dioscin and Biotransformed Fenugreek

Journal of the Korean Society for Applied Biological Chemistry volume 53, pages 470–477 (2010)Cite this article

Abstract

Trigonella foenum-graecum, commonly known as fenugreek, is widely used as a spice in India, Pakistan, and China for centuries. It has been suggested that biotransformation of the glycosides by various microorganisms increase the biological activity and bioavailability. This study aims to characterize the biotransformation of the fenugreek saponins by food microorganisms and to assess the cytotoxicity of the biotransformed fenugreek extract and dioscin which is a compound produced during biotransformation against various cell lines. The production of dioscin was confirmed by TLC and LC-MS analysis. The cytotoxicities against HT-29 colon cell line were increased after biotransformation by Aspergillus usamii, Bifidobacterium infantis, Bifidobacterium sp. Int57, and Leuconostoc paramesenteroides but decreased by Aspergillus niger. The IC50 values of dioscin against five different tumor cell line cells ranged 0.72-3.22 and 2.88-8.53 μM in 2% and 10%(v/v) FBS containing media, respectively. Real time cell analysis showed that HT-29 cells died immediately after treatment with dioscin above 7.5 μM. Dioscin at 5 μM increased sub G1 phase cells when assessed with flow cytometric analysis. Since caspase-3 activity was increased by dioscin, cytotoxicity of dioscin might be related to apoptosis. Taken together, the structure and the level of saponin components in fenugreek were differentially affected by various food microorganisms. The safety and the cytotoxicity of dioscin produced during biotransformation need to be further evaluated in vivo.

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Authors and Affiliations

  1. Department of Food and Nutrition, Seoul National University, 151-742, Seoul, Republic of Korea

    Chae Hyun Yum, Hyun Ju You & Geun Eog Ji

  2. Research Institute, BIFIDO Co., Ltd., 250-804, Gangwon-do, Republic of Korea

    Geun Eog Ji

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  1. Chae Hyun Yum
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  2. Hyun Ju You
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Correspondence to Geun Eog Ji.

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Yum, C.H., You, H.J. & Ji, G.E. Cytotoxicity of Dioscin and Biotransformed Fenugreek. J. Korean Soc. Appl. Biol. Chem. 53, 470–477 (2010). https://doi.org/10.3839/jksabc.2010.072

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  • DOI: https://doi.org/10.3839/jksabc.2010.072

Key words

  • biotransformation
  • cancer cells
  • cytotoxicity
  • dioscin
  • Trigonella foenum-graceum