Effect of Kombucha Tea on Aflatoxin B1 Induced Acute Hepatotoxicity in Albino Rats-prophylactic and Curative Studies
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Kombucha tea is sugared black tea fermented with a consortium of bacteria and yeasts which forms tea fungus (Medusomyces gisevii) for 14 days. Kombucha tea is claimed to have various beneficial effects on human health but very less scientific evidences are available in the literature. In the present study, the prophylactic and curative effect of black tea (unfermented black tea) and kombucha black tea (KBT) on aflatoxin B1 induced liver damage was studied in male albino rats by analyzing hepatotoxicity markers (aspartate transaminase, alanine transaminase, alkaline phosphatase, gammaglutamyl transpeptidase), lipid peroxidation, reduced glutathione and antioxidant enzymes (glutathione-S-transferase, glutathione peroxidase, glutathione reductase, catalase and superoxide dismutase). Histopathological analysis of liver tissue was also carried out. Results revealed that kombucha tea is more potent in preventing hepatotoxicity than unfermented black tea. The mechanism of hepatoprotection offered by KBT treatment may involve the facilitation of both antioxidant and detoxification processes in the liver.
Key wordsaflatoxinB1 Kombucha liver tea fungus
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- CPCSEA (2003) CPCSEA guidelines for laboratory animal facility. Indian J Pharmacol 35, 257–274.Google Scholar
- Habig WH, Pabst MJ, and Jakoby WB (1974). Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem 249, 7130–7139.Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265–275.Google Scholar
- Paglia DE, and Valentine WN (1967) Studies on the quantitative and qualitatative characterization of erythrocyte glutathione peroxidase. J Laboratory Clin Med 70, 158–169.Google Scholar
- Pauline T, Dipti P, Anju B, Kavimani S, Sharma SK, Kain AK, Sarada SKS, Sairam M, Ilavazhagan G, Kumar D, and Selvamurthy W (2001) Studies on toxicity, anti-stress and hepatoprotective properties of kombucha tea. Biomed Environ Sci 14, 207–213.Google Scholar
- Rastogi R, Srivastava AK, and Rastogi AK (2001) Biochemical changes induced in liver and serum of aflatoxin B, treated male wistar rats: Preventive effect of picroliv. Pharmacol Toxicol 88, 53–58.Google Scholar
- Stresser DM, Bailey GS, and Williams DE (1994) Indole-3-carbinol and a-naphthoflavone induction of aflatoxin B1 metabolism and cytochrome P-450 associated with bioactivation and detoxification of aflatoxin B1 in the rat. Drug Metab Dispos 22, 383–391.Google Scholar
- Xie B, Shi H, Chen Q, and Ho CT (1993) Antioxidant properties of fractions and polyphenol constituents from green, oolong and black tea. Proc Natl Sci Counc Republic China B 17, 77–84.Google Scholar