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Isolation of triterpenoids from the stem bark of Albizia julibrissin and their inhibition activity on ACAT-1 and ACAT-2

Journal of the Korean Society for Applied Biological Chemistry volume 53, pages 310–315 (2010)Cite this article

Abstract

The dried stem bark of Albizia julibrissin was extracted in 70% aqueous EtOH, and concentrated extracts were partitioned with EtOAc, n-BuOH, and H2O, successively. From the EtOAc fraction, three triterpenoids were isolated through repeated silica gel and octadecyl silica gel column chromatography. Based on nuclear magnetic resonance spectrometry (NMR), mass spectrometry, and infrared spectroscopy spectroscopic data, the chemical structures of the compounds were determined to be lupeol (1), betulinic acid (2), and oleanolic acid (3). This was the first report in which compounds 1, 2, and 3 were isolated from the stem bark of A. itjulibrissin. Since the NMR data for some compounds have been incorrectly or incompletely identified in previous literature, the NMR were revised on the basis of 2-D NMR experiments. Compounds 1, 2, and 3 showed inhibition activity on ACAT-1 with values of 89.3±3.7%, 61.2±3.4%, and 52.5±0.7%, respectively, at a concentration of 50 μg/mL and on ACAT-2 with values of 44.3±2.1%, 55.5±0.3%, and 22.0±2.6%, respectively, at a concentration 50 μg/mL.

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Authors and Affiliations

  1. Graduate School of Biotechnology and Institute of Life Science & Resources, Kyung Hee University, 446-701, Yongin, Republic of Korea

    Mi-Young Baek, Jin-Gyeong Cho, Dae-Young Lee & Nam-In Baek

  2. Department of Herbal Foodceutical Science, Daegu Haany University, 712-715, Gyeongsan, Republic of Korea

    Eun-Mi Ann

  3. National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology, 305-333, Daejeon, Republic of Korea

    Tae-Sook Jeong

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  1. Mi-Young Baek
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  2. Jin-Gyeong Cho
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Correspondence to Nam-In Baek.

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Baek, MY., Cho, JG., Lee, DY. et al. Isolation of triterpenoids from the stem bark of Albizia julibrissin and their inhibition activity on ACAT-1 and ACAT-2. J. Korean Soc. Appl. Biol. Chem. 53, 310–315 (2010). https://doi.org/10.3839/jksabc.2010.048

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  • DOI: https://doi.org/10.3839/jksabc.2010.048

Key words

  • ACAT-1
  • ACAT-2
  • Albizia julibrissin}
  • betulinic acid
  • lupeol
  • NMR
  • oleanolic acid