Abstract
A simple and rapid purification method of deoxypodophyllotoxin from the crude methanol extract of rhizomes of Anthriscus sylvestris was established using high-speed counter-current chromatography. From the crude extract (288.9 mg), deoxypodophyllotoxin (8.8 mg) was separated using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (7:3:5:5, v/v). The final purity of the deoxypodophyllotoxin was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
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Quan, GH., Chin, YW., Lee, HK. et al. Preparative isolation and purification of deoxypodophyllotoxin from the rhizomes of Anthriscus sylvestris by high-speed counter-current chromatography. J. Korean Soc. Appl. Biol. Chem. 53, 110–113 (2010). https://doi.org/10.3839/jksabc.2010.019
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DOI: https://doi.org/10.3839/jksabc.2010.019