Abstract
The present study demonstrated that the intracellular level of ATP was enhanced up to two-fold and the expression of ramR, a regulatory factor of aerial hyphae development, was repressed in an adenosine kinase mutant of Streptomyces lividans, substantiating that ATP-accumulation is a factor suppressing aerial hyphae development in the mutant.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- ADK:
-
adenosine kinase
- Ado:
-
adenosine
- ADP:
-
adenosine 5′-diphosphate
- AMP:
-
adenosine 5′-monophosphate
- ATP:
-
adenosine 5′-triphosphate
- EGTA:
-
ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
- HPLC:
-
high performance liquid chromatography
- SAH:
-
S-adenosylhomocysteine
- SAM:
-
S-adenosylmethionine
- SAX:
-
strong anion exchanger
- SEM:
-
scanning electron microscopy
- TCA:
-
trichloroacetic acid
- WT:
-
wild-type
References
Bang BH, Rhee MS, Kim JO, and Yi DH (2008) Purification and characteristics of tyrosine inhibitor produced by actinomycetes F-97. J Korean Soc Appl Biol Chem 51, 153–158.
Capstick DS, Willey JM, Buttner MJ, and Elliot MA (2007) SapB and the chaplins: connection between morphogenetic protein in Streptomyces coelicolor. Mol Microbiol 64, 602–613.
Chater KF and Horinouchi S (2003) Signalling early development events in two highly diverged Streptomyces species. Mol Microbiol 48, 9–15.
Chater KF and Chandra G (2006) The evolution of development in Streptomyces analysed by genome comparisons. FEMS Microbiol Rev 30, 651–672.
Ingram C, Brawner M, Youngman P, and Westpheling J (1989) xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolites controlled promoter. J Bacteriol 171, 6617–6624.
Katahira R and Ashihara H (2006) Role of adenosine salvage in wound-induced adenylate biosynthesis in potato tuber slices. Plant Physiol Biochem 44, 551–555.
Keijser BLF, van Wezel GP, Canters GW, and Vijgenboom E (2002) Development regulation of the Streptomyces lividans ram genes: involvement of RamR in regulation of the ramCSAB operon. J Bacteriol 184, 4420–4429.
Kieser T, Bibb MJ, Buttner MJ, Chater KF, and Hopwood DA (2000) In Practical Streptomyces Genetics. John Innes Foundation, Norwich, UK.
Kloor D and Osswald H (2004) S-Adenosylhomocysteine hydrolase as a target for intracellular adenosine action. Trends Pharmacol Sci 25, 294–297.
Li M (2008) Studies on the effects of extracellular ATP on Streptomyces spp. PhD Thesis, Myongji University, Yongin, Korea.
Li M, Kim TJ, Kwon HJ, and Suh JW (2008) Effects of extracellular ATP on the physiology of Streptomyces coelicolor A3(2). FEMS Microbiol Lett 286, 24–31.
Long MC, Escuyer V, and Parker WB (2003) Identification and characterization of a unique adenosine kinase from Mycobacterioum tuberculosis. J Bacteriol 185, 6545–6555.
Moffatt BA, Stevens YY, Allen MS, Snider JD, Pereira LA, Todorova MI, Summers PS, Weretilnyk EA, Martin-McCaffrey L, and Wagner C (2002) Adenosine kinase deficiency is associated with developmental abnormalities and reduced transmethylation. Plant Physiol 128, 812–821.
Morrison BA and Shain DH (2008) An AMP nucleosidase gene knock out in Escherichia coli elevates intracellular ATP levels and increases cold tolerance. Biol Lett 4, 53–56.
Nguyen KT, Willey JM, Nguyen LD, Nguyen LT, Viollier PH, and Thompson CJ (2002) A central regulator of morphological differentiation in the multicellular bacterium Streptomyces coelicolor. Mol Microbiol 46, 1223–1238.
O’Connor TJ and Nodwell JR (2005) Pivotal roles of the receiver domain in the mechanism of action of the response regulator RamR of Streptomyces coelicolor. J Mol Biol 351, 1030–1047.
Payne SM and Ames BN (1982) A procedure for rapid extraction and high pressure liquid chromatographic separation of the nucleotides and other small molecules from bacterial cells. Anal Biochem 123, 151–161.
Rajkarnikar A, Kwon HJ, and Suh JW (2007) Role of adenosine kinase in the control of Streptomyces differentiations: loss of adenosine kinase suppresses sporulation and actinorhodin biosynthesis while inducing hyperproduction of undecylprodigiosin in Streptomyces lividans. Biochem Biophys Res Commun 362, 322–326.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rajkarnikar, A., Suh, JW. & Kwon, HJ. Accumulation of intracellular ATP is a factor suppressing morphological differentiation in adenosine kinase mutant of Streptomyces lividans . J. Korean Soc. Appl. Biol. Chem. 52, 401–404 (2009). https://doi.org/10.3839/jksabc.2009.071
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.3839/jksabc.2009.071